Veterinary Advice Online - Coccidia Infection. Coccidiosis in dogs, cats and other animals.
Coccidia is a general term for a range of microscopic protozoan parasites that inhabitthe intestines and, occasionally, other bodily organs of animals and humans. Theinformation contained within this page provides information and advice on the common intestinal coccidian species (Isospora, Hammondia, Besnoitia and Sarcocystis) known to cause gastrointestinal disease, enteritis and symptoms of weight loss, dehydration, diarrhea, mucoid and bloody feces, straining to defecate and, occasionally, vomiting in puppies and kittens. If your vet has performed a fecal float on the faeces of your kitten or puppy and diagnosed your pet with coccidia or coccidiosis - this is the page for you.
The information presented in this page is detailed (but still easy to understand) because we areaiming to educate owners thoroughly about the disease, including its transmission, symptoms, treatmentand prevention, and provide owners with enough information that they might be better informed and able to troubleshoot problems with their own pets. Coccidia Contents1) What is coccidiosis?
- a basic overview of the disease and its symptoms.2) Which animals are at risk of contracting coccidia? 2a) Coccidia in dogs and cats.
2b) Coccidia in livestock and poultry.
2c) Coccidia in wildlife, including Australian wildlife.
2d) Coccidia in people. Is the disease contagious (zoonotic) to man?3) How do animals contract coccidia?
- this section contains information about where and how animals (focus is on dogs and cats) catch coccidia and the roles that stress and environmental factors play in the disease. 3a) Coccidia transmission (includes info on wildlife and livestock reservoirs of coccidia).
3b) What environmental conditions predispose to coccidia transmission and manifestation of disease symptoms?.
3c) Real-life situations that promote dog to dog and cat to cat transmission of coccidia.
3d) My pet hasn't been near another dog or cat in months - how could he get coccidia? 4) Symptoms of coccidiosis in dogs and cats - what does coccidia do to your dog or cat? This section contains the following subsections: 4a) How coccidian parasites cause disease (how they replicate and destroy cells etc.).
4b) Symptoms of coccidia: how do coccidia affect the gastrointestinal tract?
Section includes information on coccidial symptoms and secondary coccidial complications: dehydration, blood loss, secondary bacterial infections, intussusception, rectal prolapse and fading kittens and puppies. Contains great veterinary medical pictures.
4c) Chronic, recurrent coccidia infections - coccidia that keeps coming back.
4d) Coccidia diagnosed in adult dogs and cats - is it significant?
4e) Can animals be infected with coccidia and show little or no signs of disease?
4f) Sarcocystis neurona (equine protozoal encephalomyelitis), a newly emerging, feline coccidian disease.
5) How is coccidiosis diagnosed?
5a) Fecal flotation.
5b) Fecal smears.
5c) Fecal oocyst culture for sporulation.
5d) Intestinal biopsies.
5e) Additional diagnostics - when you are not certain coccidia is to blame.
6) What other diseases look like coccidiosis in cats and dogs?
7) Coccidiosis treatment
7a) Antimicrobials - coccidiostatic drugs.
7b) Antibiotics - antibacterial drugs.
7c) Intravenous fluids.
7d) Blood transfusions.
7e) Dietary support.
8) What is the prognosis for coccidiosis in canines and felines?
9) How to prevent coccidia in pets. - this section contains excellent general advice on the prevention of coccidiosis for pet owners.
10) How to prevent coccidiosis in high risk situations. - this section contains useful tips and hintsfor preventing and controlling coccidiosis infection and spread in situations with high parasite contaminationand high dog and cat numbers (e.g. breeding facilities, pet shops, boarding kennels, vet clinics).
11) How do you disinfect the environment following feline and canine coccidia contamination?
12) How do you disinfect/decontaminate coccidia-infected meat?
13) Summary and take home messages - a summary of the important points.
Please note that other closely-related, highly problematic coccidian species such as Neospora, Toxoplasma and Cryptosporidium will not be discussed here - they will shortly have their own specially dedicatedpages.
WARNING - IN THE INTERESTS OF PROVIDING YOU WITH COMPLETE AND DETAILED INFORMATION, THIS SITE DOES CONTAIN MEDICAL AND SURGICAL IMAGES THAT MAY DISTURB SOME READERS.
1. What is coccidia infection (coccidiosis)?
Coccidia are microscopic protozoan parasites that infest and damage the cells lining theintestinal walls (small intestine and sometimes large intestine) of kittens and puppies and, occasionally, adult dogs and cats. There are many species of protozoan parasite that come under the general heading of'coccidia' and which may be able to cause disease in animals. The common dog and cat speciesinclude: Isospora (Isospora canis, I. burrowsi, I. ohioensis and I. neorivolta in the dog and Isospora felis and I. rivolta in the cat);Hammondia; Besnoitia (cat only) and Sarcocystis. Wild animals (e.g. opossums, kangaroos, wallabies, birds) and livestock (e.g. calves, piglets, poultry) have their own coccidian parasite species, including: Eimeria, Sarcocystis and certain Isospora species.
No mention will be made of the closely-related intestinal protozoan parasite species: Neospora, Toxoplasma and Cryptosporidium as these organisms create very different disease syndromes with different presentations and significance to animals and humans.
The disease, coccidiosis, is contracted when kittens and puppies (cats and dogs) consume feces, faecally-contaminated soil and vegetation and (depending on the parasite species involved) the uncooked meat and organs of rodents and other wild and livestock animals, which are contaminated withinfectious coccidian organisms. The infestation that results causes damage to the lining ofthe small intestine, and occasionally the colon, sometimes resulting in symptoms of intestinal and colonic upset. Coccidiosis is a multifactorial disease in that the appearance of disease symptomsdepends not only on the presence of infectious organisms (coccidia), but on other, non-organismfactors including stress, host immune system integrity, the age of the animal host and a widerange of contributing environmental factors (e.g. overcrowding of animals, poor sanitation).Section 3 has plenty of excellent information on environmental and host factors contributing to coccidia disease presentation.
Animals that do develop symptoms of disease may present with variable degrees of: inappetence; dehydration; abdominal pain; weight-loss; mucoid, slimy (jelly-like) faeces; putrid-smelling faeces; fresh, bright red blood in the faeces; watery to mucoid diarrhea and repeated straining to defecate.Vomiting can sometimes occur, but is a less-common finding. Most dogs and cats thatpresent with clinical signs of coccidiosis are under 4 months of age (although older animalscan sometimes become symptomatically affected). Most of these affected animalsare bright and alert, despite their symptoms, and often recover without complication. Kittens and puppies can, however, sometimes become very sick from the disease (due to severe dehydration, blood loss or secondary bacterial infection and complications) and may even die without prompt and proper therapy.
2. Which animals are at risk of contracting coccidia?
2a) Dogs and Cats:
Dogs and cats - definitive hosts:
Dogs and cats of any age are able to ingest infectious coccidian parasites and have themreplicate within their intestinal tracts. Whether or not this coccidian infection actually results in any symptoms of disease (diarrhoea etc.), however, has a lot to do withthe age and immune system integrity of the animal host. Coccidian oocysts (the infectiousparasitic cysts shed in the host-animal's faeces) can often be found in the feces of older cats and dogs without these animals showing any signs of disease. Typically, coccidiosis disease symptoms are only seenin very young puppies and kittens (under 4 months old) whose immune systems are notyet mature enough to manage and control the parasitic invaders.
The kittens and puppies with the most severe infestations of coccidia (most severe signs of disease) are often those with histories of poor living conditions (e.g. poor sanitation, squalid housing) and/or high stress-levels (stress further suppresses the host immune system). Kittens and puppies that are thin and malnourished; have other intestinal diseases (e.g. parasites and worms); are living in overcrowded conditions (lots of disease spread) or that are living inconditions of poor sanitation and poor husbandry (extremes of heat and cold, draughts, lots of underweight kittens and puppies all walking around in each other's feces etc.) are most likely to suffer from the disease. Sometimes, just the stress of moving to a new home can beenough to bring out the disease symptoms in a young animal (e.g. bringing a kitten home from a shelteror pet shop can be enough stress to make it display disease symptoms). Occasionally, older dogs and cats can display symptoms of coccidiosis if placed under conditionsof high stress or if they develop other intestinal diseases or immune system suppressive disorders (e.g. cats thatcatch FIV, animals on chemotherapy or immune suppressant drugs). Animals with congenitalimmune disorders are prone to severe, recurrent coccidial infestations - this may bea reason why the German Shepherd breed is thought to be particularly prone to this parasite.
Some terminology and parasite pointers:
Because these aforementioned coccidial species (Isospora, Hammondia, Besnoitia and Sarcocystis) are able to replicate within the intestines of the dog or cat, achieve sexual maturityin these hosts and create infectious cysts that shed into the faeces, ready to infect other animals, these cat and dog hosts are termed definitive hosts. Definitive hosts are thehosts that the organism was intended for and that the organism reaches sexual maturity in.
In the case of Sarcocystis, Hammondia and Besnoitia, the cat or dog can only be infected by the parasite if it eats the meat or offal of an intermediate host(usually a herbivore or omnivore) that contains immature forms of this organism encysted in its tissues. This is termed an indirect life cycle: greater than one host species is required by theseorganisms. In the case of Isospora, the coccidian organism is able to cycle through the dog or cat population without any need for an intermediate host. Basically, dogs or cats can become infected directly through the ingestion of feces or faeces-contaminated grass and soil containing infectious Isospora parasite cysts. This is termed a direct life cycle:it is a definite-host to definitive-host lifecycle. It is for this reason that the main species of coccidial organism seen in kittens and puppies is Isospora.Its lifecycle is far more simple and direct: it can simply be caught fromeating the feces of other cats and dogs - it does not require the animal to predate onother animals (kittens and pups are too young to hunt).
The final important thing to note is thatcats and dogs have their own coccidia species that only infects them. Catcoccidia do not infect the intestines of dogs or any other animals and vice versa.
The cat (and dog?) as an intermediate host:
Sarcocystis neurona is a parasite of opossums (opossums are the definitive hoststhat shed infectious Sarcocystis oocysts into their faeces) that can infect anumber of intermediate hosts, including the domestic cat and horse. When oocysts are ingested by a cat, the immatureforms of the Sarcocystis organism replicate within and encyst within the brainand muscles of the cat. This causes brain disease and severe (often fatal) neurological signs in infected cats - see section 4f for details. Another species of Sarcocystis, dubbed S. canis, may cause liver, brain and skin disease symptoms in dogs. Itslifecycle is currently unknown.
2b) Livestock animals and coccidia:
Livestock as definitive hosts:
Various species of coccidia are known to cause severe weight loss, ill thrift and intestinal upsets in young livestock animals, particularly animals which are overcrowded and stressed and subjected to physiological stresses(e.g. excessive growth-rates, frequent pregnancy and lactation, weaning) and poor standards ofhygiene and sanitation. Most species of livestock animal (horses, deer, rabbits, cattle, pigs, poultry, goats and sheep) have their own specific species of intestinal coccidia: Eimeria species tend to be the main species implicated in livestock infections, but Isospora has also been implicated as an intestinal pathogen in pigs.
Pigs, cattle and chickens (and other poultry) raised in intensive farmingsituations, are particularly subject to a large variety of stressors (e.g. transportation, malnutrition, other parasites) and environmental conditions facilitating the spread of coccidiosis and, as a consequence of this, coccidia are a major parasitic nuisance affecting these farming systems. Clinical signs (watery to mucoid or bloody diarrhoea, tenesmus, dehydration, weight loss, even death) are similar to those seen in the cat and dog. Millions are spent every yearcontrolling coccidia in pig, cattle, goat and poultry operations and tonnes of the antibioticmedications used to control the parasites are put into the feed of such animals, trying to prevent themfrom becoming sick and losing weight. This has, naturally, lead to public concerns about the developmentof antibiotic resistant bacteria in farming operations (through antibiotic overuse)and concerns about potentially-toxic antibiotics entering the human food chain.
Some terminology and parasite pointers:
Because these coccidial species (Eimeria, Isospora suis) are able to replicate within the intestines oflivestock animals, achieve sexual maturity in these hosts and create infectious oocysts that shed into the faeces, ready to infect other animals, these livestock hosts are termed definitive hosts. They are the hosts that the organism was intended for and that the organism reaches sexual maturity in. These intestinal coccidia species (Eimeria, Isospora) are able to cycle through the livestock population without any need for an intermediate host: i.e. cattle can become infected directly through the ingestion ofcattle feces or cattle-faeces-contaminated grass and soil containing infectious parasite cysts.This is termed a direct lifecycle. The final important thing to note is thateach livestock animal species has its own coccidia species that only infects it(i.e. each species of coccidian is host-specific). Pig coccidians do not infect the intestines of cattle or any other animals; cattle coccidia only infect cattleand turkey coccidia only infect turkeys and so on. Even sheep and goats differ enoughfrom each other that goat coccidia do not infest sheep and vice versa.
Livestock as intermediate hosts:
In addition to intestinal coccidiosis, livestock animals may also become infected withcoccidial species that typically replicate and achieve sexual maturity within the intestines ofother, carnivorous, non-livestock animals, including dogs and cats. When livestockingest grass that has been contaminated with the coccidia-infected faeces of cats or dogs (and other carnivores), these parasites can hatch out in the livestock animal's intestines and migrate through thewall of the intestines into the animal's visceral organs (e.g. abdominal lymph nodes, walls of theblood vessels, liver, muscles and brain). The invading organisms replicate withinand damage the cells of these internal organs until the livestock animal's immunesystem responds and suppresses this replication, forcing the organisms to hide out in walled off cystswith those tissues. These organisms remain living and can lie dormant, hidden within the animal's organs and muscles, for many years. Symptoms of this intermediate host infection may not be observed unless the parasite is a particularly aggressive species (e.g. Sarcocystis tenella in sheep, S. cruzi in cattle); the parasitedamages a vital region of the host's body (e.g. a major blood vessel, the brain)or the livestock animal's immune system response is particularly weak and the parasite is permitted to run rife and uncontrolled through the organs, creating extensive damage. If the meat (muscle)or offal (internal organs) of an infected livestock animal is later fed, uncooked, to thecorrect carnivore definitive host species, the livestock tissue cysts will reactivate,hatch out in that carnivore's gut and replicate in the intestines of that animal, thereby causing intestinal infection (+/- clinical symptoms of disease).
Some terminology and parasite pointers:
In this aforementioned situation, the cattle or livestock animal is termed anintermediate host because the parasite needs this livestock host as part of its lifecycle, but is unable to attain sexual maturity in this host. The carnivore (dog or cat etc.)is the definitive host in this situation because the coccidia are able to replicate within the intestines of this animal, achieve sexual maturity in this animal and create infectious cysts that are shed in the faeces.
The final important thing to note is that many species of coccidian parasite are specific to only one type of intermediate host animal (e.g. Sarcocystis. tenellaon infects sheep and S. suihominis only infects pigs) and one or sometimesa couple of definitive hosts within the same carnivore family (e.g. S. tenellaonly infects canids, S. suihominis only infects primates). Consequently, if an intermediate host animal consumes a fecally-shed coccidian oocyst that it is not adapted to (e.g. if a pig ate an S. tenella cyst, intended for a sheep) or a definitive host carnivore consumes meat containing a parasitic tissue cyst that it is not adapted to (e.g. if a primate ate sheep meat containing S.tenella), nothing will happen. The cysts will travel right through the intestines and out into the faeces and cause no issues at all. Only the right definitive host and intermediatehost species can take part in the coccidian lifecycle of each coccidian species.
2c) Wildlife animals and coccidia:
Wildlife - definitive hosts:
A massive range of wild animal species including: Australian macropods (e.g. kangaroos, wallabies), rabbits, hares, opossums, wild livestock species (deer, cattle etc.), frogs, platypus, echidnas, seals, wombats and wild bird species (e.g. birds of prey, pheasants, wild ducks, geese and poultry-types), have their own species of coccidia which replicate within their intestines, sometimesproducing signs of intestinal disease. As occurs with the other, aforementioned, domestic and livestock animal species, the coccidial species affecting wild animals are very host specific: they will only replicate and cause disease in a specific wild animal definitive host species.
Disease symptoms, when they occur, tend to occur most frequently in young animals, particularly those that are under conditions of high stress, are malnourishedor are infected with other diseases (e.g. other intestinal parasites) or subjected to poor environmentalconditions. For example, coccidiosis is very common in baby, hand-raised marsupials (e.g.baby wallabies, possums and kangaroos) because they are suffering from the severe stresses of altered diet (sometimes even an inappropriate diet), human handling and loss of their mother.Severe clinical infestations are also commonly encountered when the juvenile animal first starts to graze pasture (e.g. around 10 months of age in wombats): it is thought that these animals are being exposed to large amounts of coccidia in the pasture, but have not yet developed good enough anti-coccidial intestinal immunity to deal with it. Consequently, the organisms are permitted to replicate freely and these animals develop symptoms of disease. Some wild animal species (e.g. frogs) seem to suffer very little from their enteric coccidial infections, rarely showing any symptoms of disease, whereas other species(seals, marsupials, echidnas) may often develop severe disease, even death. For example, echidnas often die from a form of coccidiosis that disseminates from their intestines throughout theirinternal organs.
Wildlife - intermediate hosts:
Similar to the situation described in the livestock section, many species of wild animals (particularlyherbivores and omnivores) are intermediate hosts for a range of carnivore coccidial species. As occurs with the livestock coccidian types, these wildlife coccidia are very host-specific, only encysting and going dormant within the muscles and organs of the correct wild animal species. The coccidian parasite only completes its lifecycle when the intermediate host wild animal is consumed by the correct carnivorous animal adapted to play definitive host to that coccidial organism. Often this definitive host animal is a wild carnivore (fox, wolf, wild cat, dingo, coyote) found in the same environment as the wild intermediate host and the lifecycle takes place completely within the wild (sylvatic) environment, without any involvement from people, their carnivorouspets or their livestock.
2d) People and coccidia - is the disease zoonotic (infectious to man)?:
Most of the enteric canine and feline coccidian species discussed on this page are not directly orindirectly transmissible to humans. People should not be worried about contractingIsospora, Hammondia, Sarcocystis or Besnoitia from their pets.
Humans do have their own specifically-adapted species of coccidian organisms thatcan infest them and cause disease within them, however, these tend to come frominteractions with other infected humans, not from interactions with infected pets. Certain speciesof intestinal coccidia (e.g. Sarcocystis suihominis, S. hominis) can infest man through the consumption of raw or severely under cooked pork and beef products.
Closely-related protozoan organisms such as Toxoplasma gondii and Cryptosporidiumparvum are capable of infecting people (zoonoses). Sometimes, these infectious organismsmay cause severe disease symptoms and even death in infected humans. People may contractthese organisms from domestic pets and from the consumption of certain under cooked meat products.These zoonotic organisms will not be discussed further on this particular page, but willhave their own specifically dedicated pages in the near future.
3. Coccidia transmission: how do dogs and cats contract coccidia?
From here on in, we shall leave the livestock and wildlife animals behind and focusour discussion on coccidia and coccidiosis in the dog and cat. This section contains informationabout coccidia transmission in dogs and cats and the role that environmental and host-relatedfactors play in contributing to and exacerbating the symptoms of disease.
3a) Coccidia transmission in Dogs and Cats.
A number of species of Isospora are known to infect our domestic pets, in particular:Isospora felis and Isospora rivolta in the cat and Isospora canis, I. neorivolta, I. ohioensis and I. burrowsi in the dog. This parasite species (Isospora) has no intermediate host requirement and is able to spread from cat to cat and dog todog via direct fecal-oral transmission. It is highly contagious. Kittens and puppies (and older animals)become infected if they consume Isospora-contaminated feces from other cats or dogs and may even reinfect themselves through the consumption of their own Isospora-infected droppings. This is one reasonwhy owners should not let their dogs and cats consume the stools of other animals.It is also a reason why dog and cat food and water bowls should not be placed in a positionwhereby the food and water could become contaminated with fecal material (e.g. pets stepping into food and water dishes with their poop-covered feet) and, subsequently, consumed.
An important thing to note is that the Isospora oocysts shed into a pet's feces are not immediately infective to other animals: they need to mature slightly and develop into an infective form before they can infect another dog or cat. This maturation processtakes at least 8 hours (minimum), which is important to knowbecause much control of the disease can be achieved simply by cleaning out fecesregularly (two to three times daily), prior to the maturation of the cysts.
See the image on the right: This is an Isospora canis oocyst from a dog with coccidiosis. Thecentral ball of cells within the oocyst is in the process of dividing. This fecal sample was only 30 minutes old and the cysts were already starting to divide and mature towards an infective state.
The main species of enteric Coccidia infecting kittens and puppies is Isospora:Another thing to be aware of, when it comes to Isospora-infected faeces, is that faecesinfected with this organism do not have to look abnormal and nor do they haveto come from a kitten or puppy. Isospora is not only foundin obviously-diseased, slimy, bloody-looking diarrhoea specimens (e.g. the image on the right). The organism can be found in perfectly formed, normal-looking faeces produced by subclinically infected kittens,puppies and adult animals. Coccidia carriers (animals that have the infection and are sheddingthe organisms in their faeces, but which show absolutely no symptoms of disease) are very commonin the dog and cat population and their faeces, though normal-looking, can be highly infective to other, more-susceptible animals.
The infective cysts of Isospora are somewhat resistant to the effects of sunlight, desiccation, freezing and numerous disinfectants and they can persist in the environmentand in dog and cat facilities for a very long period of time (many months), long after the visible fecesthemselves have broken down and disappeared. Consequently, wherever there has been coccidia-contaminated feces in the past (indoors or outdoors), there are potentially infectious coccidia organisms still remainingin the environment. Just because you can't see any droppings does notmean that the surfaces: pavement, concrete, dirt/soil, lawn, cages, food bowls, grooming equipment, ground or vegetation are not infective to your dog or cat. The puppy that you have just taken to the park could walk across contaminated ground and come home, lick its feet, and get infectedby parasitic oocysts in microscopic faeces that you didn't even see!
Dogs and cats can also ingest infectious particles when they lick the shoes, hands or clothesof a human that has been in hands-on contact with an affected animal. Those hands and clothes may look clean and may have even been washed, but infectious coccidia cysts might still bealive on them. Cats and dogs which bite and lick the fur of infected animals may also contractthe organism. Animals spread protozoan cysts onto their fur through rolling in or lying in feces or through licking their feces and then licking their bodies (the cysts are transferredfrom the tongue of the animals onto the coat).
In addition to direct fecal-oral transmission (the direct lifecycle), it is also possible for Isospora toestablish an intermediate host life-cycle (termed an indirect lifecycle). Infective Isospora cysts, shed in the fecesof infected dogs or cats, can be consumed by a range of mammalian intermediate hosts, includingvarious rodent species (i.e. perfect prey animals for the cat or dog), when they eat grainor grass that has become contaminated with dog or cat feces. Once consumed, these Isospora parasites hatch out in the rodent's (or other animal's) intestines and migrate through the wall of the intestines to the rodent's visceral organs (abdominal lymph nodes, lungs, liver, muscles, brain etc.). The invading Isospora organisms do not replicate within the cells of these internal organs like other coccidial species do. They justhide out singularly in walled-off cysts within the tissues - here they can lie dormant, hidden within the animal's organs, for many months. Because the organisms do not replicatewithin the rodent, symptoms of the infection in the rodent intermediate host will generally not be observed. If the meat (muscle) or offal (internal organs) of the infected rodent is later consumed by a predatory cat or dog, then (provided that the predatory carnivore is the correct definitive host species for the particular coccidial cyst species) the walled-off cysts will reactivate and hatch in that carnivore's gut.The reactivated organisms will replicate in the small intestine and colon of the dog or cat, thereby causing intestinal infection (+/- symptoms of disease).
In the case of the Hammondia, Besnoitia and Sarcocystis species which infect the dog orcat intestine, there is no direct, fecal-oral life-cycle. These organisms can only be contracted by a dog or cat through the consumption of uncooked meat or organs from an infectedintermediate host. Depending on the coccidial species involved, intermediate hostanimals include: rodents (esp rats and mice), rabbits, camels, cattle, sheep, pigs, buffalo, deer, goats, horses,poultry and, potentially, many species of wild herbivores and omnivores (e.g. marsupials). Basically, any kind of uncooked meat or offal could harbor infectious parasitesthat might infect your dog or cat, should you choose to feed your pet these foodstuffs.
3b) Environmental and host factors that contribute to coccidia transmission in dogs and cats.
Given that Isospora (the main coccidia type commonly found in puppies and kittens)is most commonly transmitted through the direct consumption of infected feces and via faecal contamination of food and water bowls and the general environment (e.g. grass, soil,cages, runs etc.), it follows that most dog to dog or cat to cat infections will occur when large numbers of cats or dogs are placed in enclosed conditions, in very close proximity to each other. The larger the number of animals in the one area, the morelikely it is that at least one or more of these animals will be shedding infectious particlesinto their faeces, which can subsequently infect the others. Direct fecal-oral contactis particularly likely if animals are all placed in the one enclosure together(often litters of kittens or puppies will be placed in the one cage or run together,not in individual cages, thereby promoting the spread of coccidia among them) or iffecal run-off from one cage or enclosure is permitted to leak over into adjoiningruns and cages, thereby carrying infectious disease particles to a whole new group ofanimals.
Compared to the direct, fecal-oral, route of coccidial transmission, described above, the role that predation and the consumption of intermediate host tissues plays in the transmission and manifestation of the disease is much less significant. Given that very young animalsare the ones most likely to show clinical symptoms of disease but the least likely to hunt for prey, it is unlikely that very young kittens and puppies are going to contract symptomatic coccidiosis as a resultof their own hunting activities. The most likely source of intermediate host infection for these young, susceptible animals would come as a result of a bitch or queen regurgitating raw meat, offal or prey tissues to her litter. The consumption of intermediate host tissues andconsequent infestation with coccidian organisms is much more likely to occur in olderanimals (cats and dogs that regularly hunt or fossick for carrion, hunting dogs fed on the offal and meat of their kills, adult dogs fed a bones and raw food and meat diet (BARF diet) and stray or feral animals forced to hunt for their own survival), however, given the role that age, immunity and stress plays in the manifestation of disease symptoms (see following points), it is unlikely that many of these infectedadult animals will actually go on to display symptoms of disease (unless there is something very wrong with their immunity). Additionally, since many of thesecoccidian species (Isospora excluded) actually require an intermediate host as an obligatory (essential) part of their lifecycle, this sub-clinical infestation of the adult animal is unlikely to pose a risk to the young and susceptible animalsthe adult animal associates with.
Author's note: Isospora could potentially enter a facility through the consumptionof undercooked meat or predated-upon rodents by an adult animal. The parasite could thenspread rampantly through the entire facility because of the direct fecal-oral lifecyclethat Isospora is capable of. Unlike the other species mentioned, Isospora isnot reliant on intermediate hosts to complete the lifecycle and spread the infection.
Whether or not an animal infected with Isospora or any of the other coccidianparasites actually goes on to develop symptoms of disease largely depends on the integrity of the animal's immune system, the health of its intestinal tract and the number and virulence of the organisms ingested. From an animal husbandry viewpoint, conditions with high fecal contamination levels; poor sanitation; moderate to high humidity levels(moist air); warm temperature levels and conditions where animals are frequently exposed toextremes of cold, heat, humidity (excessively wet or dry air) and draughts; poor nutrition or changes in nutrition; animal overcrowding; physiological stressors (lactation, pregnancy, weaning, growing); other diseases; excessive exercise fatigue and transportation all predispose tococcidia manifestation and spread.
High fecal contamination levels and poor sanitation:
High levels of fecal contamination in an animal's environment obviously facilitate the spread of any fecally-transmitted diseases. In the case of coccidiosis, the chanceof a susceptible animal contracting the organisms increases with the amount of time that the faeces are left lyingaround: the longer the faeces remain in the environment, the more oocysts are able tomature and reach their infective stages. High levels of faecal contamination also playa significant role in the manifestation of coccidia symptoms because, one, poor sanitary conditionscause stress to the animals that must endure it (stress suppresses the immune system'sdefenses) and, two, poor sanitary conditions promote the spread of other intestinal diseasesincluding bacterial infections and other enteric parasites (e.g. worms). These otherinfections damage the intestinal wall, making the intestine less able to resist the effects ofthe invading coccidia, and, two, these additional infections take up some of the attention of the animal's immune system defenses, diverting themaway from fighting the invading coccidia (thus signs are more likely to be seen).
Poor nutrition (which includes both malnutrition and incorrectly-balanced nutrition),has a huge role in the manifestation of coccidial disease symptoms. Goodnutrition is vital to intestinal health and maintaining the integrity of the lining ofthe intestinal tract. Poor nutrition leads to a breakdown in the alimentary tract'sstructure and alters the intestine's defenses and motility, thereby making it less able to resist the effects of the invading coccidia. Poor nutrition also resultsin a weaker immune system that is less able to fight coccidial diseases: immune cells requiregood nutrition in which to multiply and function. Animals that are starving are morelikely to try to hunt for themselves, leading to the increased consumption of intermediate hostsand encysted coccidia. Additionally, animals that are starving or that are deficientin certain nutrients are more likely to ingest grass, vegetation, soil and even thefeces of other animals, leading to the increased uptake and consumption of coccidialorganisms (the more organisms ingested, the greater the chances of that animal showing clinicalsigns of disease).
High air humidity levels and warm temperatures:
High air and ground humidity levels increase the risks of coccidial transmission because these organismstend to survive longer (hang around longer) in wet, humid environments. Temperaturealso plays an important role: coccidial oocysts tend to mature to their infectious stagesfastest in environmental temperatures ranging between 20-37 degrees Celsius (Greene CE).The faster the oocysts mature and become infective, the more likely it is that large numbers of infectious coccidial organisms will be ingested by susceptible animals (the more organisms ingested, the greater the chances of that animal showing clinical signs of disease).
Overcrowding of animals:
Overcrowding promotes the rapid spread of any infectious disease from animal to animalbecause there is a much greater likelihood of susceptible animals being placed into closeproximity with infected, pathogen-shedding animals. In the case of coccidia, overcrowdingis of particular concern when lots of stressed kittens or puppiesfrom all different backgrounds (some infected, some susceptible) are put into runs or cagestogether or when older animals (possibly sub-clinically carrying and shedding organisms)are put into runs or cages with young, susceptible animals (the older ones infect the younger ones). Overcrowding promotes stress, which suppresses the immune system responses of susceptible animals, and overcrowding makes it difficult for facility operators to identify the sick animals and to find a place, sufficiently out of the way, to isolate the sick once they have been identified.
Conditions of extreme or frequently fluctuating heat and humidity, as well as overcrowded conditions; unsanitary conditions; draughty conditions; changes in environment;transportation; exercise fatigue; weaning; pregnancy; lactation; the presence of other diseases (e.g. parasites and other illnesses) and conditions of inadequate or ever-changing nutrition all contributeto coccidiosis manifestation and spread. This is because all of these conditions result in high levels of animal stress: stressed animals have poorer immune systems and, as a result of this, are more likely to show signs of infection.Even adult animals, when placed under enough stress, can develop enough immune suppressionto display clinical signs of coccidiosis (disease symptoms normally affecting the young).Stressed animals, in addition to being more likely to show signs of disease, are alsomore likely to shed larger numbers of infectious cysts into their immediate environment(the lack of immune system defenses allows the organisms to breed freely and producegreater numbers of infectious oocysts). High levels of oocyst shedding pose a much greater risk of infection to other susceptible animals.
Aside from stress, there are many other causes of immune suppression that can affect young or adult animals. These include genetic immune system disorders (seen from ayoung age) and acquired immune system depression (e.g. immune mediated diseases, immunesuppressant drugs, chemotherapy, cancer, diabetes mellitus, Cushing's disease, infectious immunosuppressant diseases, bone marrow disorders). Individual animals afflicted with these disorders are more likely toshow clinical signs of coccidiosis and more likely to shed large numbers of infective coccidial oocysts into their faeces.
3c) Real-life situations that promote dog to dog and cat to cat transmission of coccidia:
Obviously, some environments are more risky for coccidial infection and transmission than others. Environments that are likely to have had coccidia in the past (and are thus at higher risk of still containing the infectious particles) include pet shops, shelters, breeding facilities, 'puppy farms' (backyard breeders), pounds, grooming facilities, dog clubs and, ahem, veterinary clinics.
1) Boarding kennels and catteries:
Kennels and catteries often house large numbers of dogs and cats in close proximity; these animals are often stressed (which lowers their immunity to disease) and the facilities are often designed to be of a more enclosed, space-conserving, compact structure, rather than an open-planned, spacious structure. There is a much greater chance of fecal material and infectious disease particles spreading between cages thatare closely-aligned, both through direct animal contact (face-licking etc.) and fecal run-off.The one thing in their favor is that boarding kennels and catteries do not often board extremely young animals, thereby removing the most susceptible individuals from consideration.
2) Pounds and shelters:
This is probably the worst situation for coccidia transmission. Susceptible animals are oftenkept in overcrowded conditions, in very close proximity to each other; puppies and kittensof different backgrounds are often placed in cages together because of insufficient space; conditions are often unsanitary (depending on the shelter or pound in question); animals are often very stressed and of poor nutritional status (this lowers their immunity to disease) and their vaccination and worming history is generally very poor (the animals may have otherintestinal viruses and parasites, increasing their susceptibility to coccidial disease). In addition to this, young puppies and kittens (the most susceptible animals) make up a huge proportion of shelter and pound accessions - litters of unwanted pups and kittens are dumped at such facilities by irresponsibleowners every week of the year.
3) Pet Shops:
As far as coccidia transmission goes, pet shops often do not rate that much above pounds andshelters. Pet shops often keep very young puppies and kittens in overcrowded conditions, in very close proximity to each other; puppies and kittens from different backgrounds are often placed in cages together (increasing disease spread between 'clean' and susceptible animals); conditions are sometimes unsanitary (depending on the pet shop in question) and animals are often very stressed and of poor nutritional,vaccinational and worming status. Sadly, many pet shop pups and kittens are poorer quality animals that have been offloaded by backyard breeders or irresponsible pet-ownerswho have 'ended up with a litter' - this makes their quality, immune status and background(including worming and vaccination status) an unknown factor. In addition to this, young puppies and kittens (the most susceptible animals) make up the vast majority of pet shop sales (thosecute faces and fluffy bodies make for an ideal 'impulse buy').
4) Breeding facilities:
Vaccination, worming and parasite control tends to be adequate among most breeding populations. Coccidia, if it does manage to get into a breeding colony, is likely to spread rapidly throughout the breeding facility, however, because of the number of animals located in close proximity to each other; the number of underage, highly-susceptibleanimals around (newborn puppies and kittens etc.) and the high levels of stress that can occurin bitches and queens who are pregnant and lactating and in pups and kittens that are being weaned.
3d) My pet hasn't been near another dog or cat in months - how could he or she get coccidia?
Having read up to this point, the numerous reasons for this should now be pretty self-explanatory, however, I will group those reasons together in this section for completeness. The followingpoints describe ways in which clinical signs of coccidia infection may occur in pet animalsthat have not been near another cat or dog in months.
1) Reactivation of coccidia in a carrier patient:
Coccidia are able to replicate within the intestinal tracts of all dogs and cats(young or adult), however, they only tend to create symptoms of disease in very young animals. It is not uncommon for animals that have been infected (older cats and dogs or young animals which have recoveredfrom a clinical bout of the disease) to develop a 'harmonious' and balanced co-existencewith the coccidian parasites infesting their intestines (a situation termed premunition).These animals have generally developed enough immune resistance to the organisms that theorganisms do not cause symptoms of disease in them, but not enough immune resistance that they areable to completely clear the organisms from their intestines. These animals (termedcarriers) can shed coccidia in their faeces for long periods of time (months to years),showing no symptoms of disease because organism and host are in harmony. If, however, the host suffers from any form of immune suppression (including stress-induced immune suppression) or intestinal dysfunction (e.g. ulcerative intestinal diseases, other parasites, bacterial infections), this delicate balance of host and parasite can be broken. The coccidia will reactivate and breed profuselywithin the host animal's intestines, creating symptoms of disease in an animal that might not haveseen another dog or cat for months.
Interestingly, most antibiotics and coccidiostats used in coccidia therapy do notdestroy all of the coccidial organisms. Most of them simply kill enough of the organismsthat they won't decimate the bowel, thereby buying time for the immune system response tokick in and establish this state of host-parasite balance and neutrality. Animalstreated in this way will 'seem' to have recovered from the disease, but more often than not, they will simply have converted to a non-clinical carrier state. Consequently, if these hosts then go onto develop any form of immune suppression or intestinal dysfunction, there is every likelihoodthat this delicate balance will be broken. Again, the coccidia will reactivate and breed profuselywithin the host's intestines, creating symptoms of disease in an animal that might not haveseen another dog or cat for months. When this occurs, pet owners will often blame thevet for giving the wrong antibiotics to their pet (or not treating the correct disease), when thereal problem is the underlying nature of the coccidial organism (its tendency to persist despite treatment)and the presence of some underlying stress or disease factor that keeps upsettingthe balance.
2) Coccidia persistence in the environment:
As mentioned previously, coccidian parasites are quite resistant to environmentalconditions such as desiccation, heat and cold and are able to persist in the environmentand on clothing and fomites (dog dishes and water bowls) for long periods of time. Your animal can contract the highly contagious organisms from ingesting infected feces, soil and vegetation and from licking fecally-contaminated clothing and fomites without ever going near another dog or cat. Contracting the disease can be as simple as eating poo in the park.
3) Ingestion of intermediate hosts:
Certain species of coccidia, including the highly-contagious Isospora organisms, areable to be transmitted to dogs and cats following the ingestion of the uncooked meat (muscles)and offal (organs) of infected intermediate hosts: various livestock, rodent andwild animal reservoirs. Dogs and cats can consume these tissues as a result of their ownhunting activities (e.g. cats can catch coccidia through their mousing activities) and foragingactivities (roaming dogs may eat rotting carcasses) or they may be fed these tissuesby owners who are unaware of the potential for raw-meat disease transmission (e.g.hunters who feed their pets raw offal, pet owners who subscribe to a 'natural' raw meatand offal diet). These dogs and cats do not need to meet another dog or cat in order tocontract the disease.
Interestingly, in the case of Isospora, the animal can contract the organism fromeating coccidial cysts in raw meat and offal meals, but then go on to reinfest itselfover and over through the consumption of its own infected fecal materials (fecal-oral route). This can result in dogs or cats developing significant coccidial parasite burdenswithout ever seeing another dog or cat.
4. Symptoms of coccidiosis in dogs and cats - what does coccidia do to your pet?
As mentioned in the opening sentences, coccidiosis is a disease caused by certain species of microscopic protozoan parasites that is characterized by small intestinal and (sometimes) colonic disease symptoms, including: watery to mucoid (custard-consistency) diarrhoea, fresh blood in the stools, straining to defecate (tenesmus), flatulence, dehydration, blood loss (anemia), weight loss, weakness and, sometimes, abdominal pain and vomiting. The following discussion is mostly for those of you who are interested in how these protozoan parasites infect the gastrointestinal tract of the dog and cat and create the coccidiosis symptoms observed. Understanding how the infectious disease organisms work is useful because it aids your understanding of why the various symptoms occur; what complications can develop; how the disease is spread and what treatments are available.
This section will only focus on the replication of coccidia species within the small intestine and colon of the dog and cat. These animals are, after all, the main animals that actually present to small animal veterinary clinics with symptoms attributable to coccidial infection. Aside from Sarcocystis neurona (see section 4f), which does infest intermediate host felines as part of an indirect lifecycle, coccidia infections in intermediate host animals shall not be discussed further. In the case of coccidial infections (Eimeria etc.) that do infest the intestines of wild and livestock animal definitive hosts, for ease ofsimplicity, these definitive host wild animal and livestock infestations can be thought of as being similar to that which occurs in our pet dogs and cats.
4a) How coccidian parasites cause disease (how they replicate within and destroy cells).
1. The coccidia stages infective to the dog and cat - what they are and how they form:
The stages of a coccidian organism that are infectious to a dog or cat definitive host are those that are present within an oocyst or that are encysted within the muscles or organcells of an intermediate host.
Infectious oocysts - what they are and how they become infectious:
An oocyst is the highly infectious,environmentally resistant form of a coccidian parasite that is shed into the feces ofa definitive host and which can be subsequently ingested either by a similar speciesof definitive host (direct lifecycle as seen with Isospora) or a different speciesof animal: an intermediate host (indirect lifecycle as seen with Isospora, Hammondia, Besnoitia andSarcocystis). Before an oocyst can become infectious to any other host animal, definitiveor otherwise, it needs to mature. The oocyst undergoes maturation in the environment:its internal cells divide and differentiate many times (like an embryo), eventually forming several sporocystscontaining several sporozoites. Sporocysts are sub-compartments encased within thetough, outer shell of the oocyst and sporozoites are independent, living, infectious protozoan organisms, encased inside the sporocyst compartments. The number of sporocysts and sporozoites that form, encased within each oocyst,varies depending on the species of coccidian: mature, infectious Isospora oocystshave 2 sporocysts, each containing 4 infectious sporozoites inside them, whereas infectiousHammondia oocysts contain 8 sporozoites with no sporocysts (compartments) to contain them.
Author's note: Sarcocystis matures inside the definitive host's intestines. It is mature and infectiveas soon as it exits the host in the droppings.
Infectious tissue cysts in the intermediate host - how they form:
When a mature, infectious oocyst is eaten by an intermediate host animal, the tough shell of theoocyst breaks down in the animal's intestines, along with the internal compartments (the sporocysts). This allowsthe infectious sporozoite organisms to break free. The individual protozoansporozoites (each free-living and migrating independently of the other sporozoites) migrate through the wall of the intermediate host's intestines, travel throughout the animal's body and invade the cells of various organs and muscles (the liver cells, muscle cells, blood vessel cells and brain cellsof the intermediate host are favored). When a sporozoite invades an animal cell, it multiplies rapidly within that cell by undergoing asexual reproduction (simple cell division). Hundreds of parasite protozoan clones are produced as a result of this asexual replication, all clustered together inside the single animal cell. When viewed under a microscope, they look like a ball of banana-shaped organisms all gathered within a cell. The individual, rapidly-dividing protozoans, within this cluster of organisms, are now termed tachyzoites instead of sporozoites (they are individual protozoans that replicate fast - tachy means fast). When the multiplying tachyzoite organisms inside the cell become too numerous, the host animal cell will rupture and die, releasing all of the organisms into the surroundingtissues. The individual, free-living protozoan organisms (still termed tachyzoites or, alternatively, merozoites), swim away independently, invading more cells and replicating rapidly inside those cells (forming more tachyzoite organism clusters) and continuing the cycle of infection and protozoan replication.
This rapid cycle of organism replication and cell-death is only stopped when one of two things happens:
1. The animal's immune system responds to the invasion, killing all the freely-migrating merozoites/tachyzoites it finds. The only way that the protozoan organisms are able to avoid this immune system attack is by hiding out inside of the animal cells in their clustered forms. They alter their activity, only replicating very slowly and only to the point whereby there are manyprotozoans in the cluster, but not enough to destroy the protective host cell shielding them.The replicating organisms don't want to be exposed to the immune system attack that would occur were they to destroy their protective host cell. These slowly dividing protozoans, lying dormant within the cells of the host animal, are now termed bradyzoites (brady means slow). They are essentially the same as the tachyzoites except that they replicate very slowly and go dormant inside of animal cells, (tachyzoites, in contrast, replicate rapidly in cells and aggressively destroy host cells). These bradyzoites may reactivate, increase their activity and resume rapid, tachyzoite-type replicationwithin the intermediate host's cells if the host's immune system fails.
2. The parasite fulfills its maximum number of tachyzoite (rapid) replications.Many species of coccidian are programmed to only undergo a fixed number ofrapid (tachyzoite) cell-invasion and asexual replication repetitions, after which they automatically reducetheir activity and revert into the dormant, slow-growing, tissue-encysted bradyzoite forms. These can only reactivate and continue their life cycle after being ingested by a definitive host.
Either way, the end result of this process is the formation of clusters of bradyzoites within the tissue cells of the intermediate host animal. These bradyzoite clusters are the infectious muscle and organ 'cysts' that can infect a dog or cat (definitive host)when it eats the muscles and organs of an intermediate host. In many coccidian lifecycles, only the dormant or slowly-replicating bradyzoite forms are able to infect dogs and cats, whereasthe similar-looking, but rapidly-dividing, tachyzoite forms are not infectious to these definitive hosts.
2. What happens when a dog or cat eats an infective coccidial stage (how symptoms are created).
When a dog or cat ingests the infectious, mature-stage oocysts of a coccidian organism, or ingests infectious bradyzoite-containing organs or meat of an intermediate host animal, the oocysts or bradyzoite-cysts travel through the gastrointestinal tract to the small intestineand sometimes the colon. When the parasite cysts/oocysts reach these regions, they 'excyst' (hatch). In the case of ingested oocysts, the rigid outer wall (shell) of theorganism breaks down, along with the internal sporocyst compartments, releasing the infectioussporozoites into the intestinal fluids. In the case of ingested bradyzoite cysts, the host tissues surrounding the 'ball of organisms' breaks down in the intestinal digestive fluids,releasing the individual organisms (now termed merozoites instead of bradyzoites) into the digestive fluids.
The infectious organisms (sporozoites from an oocyst or merozoites from a tissue cyst) invade the epithelial cells (the surface layer of cells) lining the walls of the intestines.They also enter the intestinal cell-layers deep to these - the lamina propria (LP) cells.When the individual protozoan organisms invade these intestinal-lining cells, they replicate asexually inside of them, similar to the situation that was described in the intermediate host tissues, forming clusters of rapidly-multiplying intracellular protozoans. When these organisms multiplying inside of the intestinal cells become too numerous, the host cells rupture and die, releasing all of the newly-created organisms into the surroundingtissues and into the intestinal tract. These individual protozoan organisms (termed merozoites), swim away independently, invading more intestinal-lining cells, replicating rapidly inside these cells and continuing the cycle of infection and protozoan replication. This process, whilst it is occurring, causes massive amounts of intestinal cell damage, resulting in the clinical symptoms seen in puppies and kittens with coccidiosis (see section 4b on symptoms).
As mentioned in the intermediate host section, many coccidians are only able to undergoa fixed number of cell-invasion and asexual replication repetitions. This is true for the replicationsituation that occurs within the definitive host's intestinal cells too. In this situation, however, instead of the final-replication merozoites remaining in a dormant, clusteredbradyzoite form (as would occur in the intermediate host), the final-replicationmerozoite forms in the intestinal cells of the definitive host set about producinginfectious, fecally-distributed oocysts.
3. How the infectious oocysts are created.
Following the final asexual replication phase, the merozoites produced are released intothe intestines (or local intestinal tissues). Each individual merozoite invades an intestinalcell but, instead of replicating asexually like it did before, it differentiates (matures) into either asingle, very large, female merozoite (termed a macrogamete) or subdivides itselfinto many, much smaller, male merozoites (termed microgametes). These microgameteshave swimming 'tails' termed flagellae (much like human sperm cells do) and, once mature, theyexit their host cell and swim over to other intestinal cells, hunting for mature femalemacrogametes. Upon finding one, the male microgamete enters the intestinal cell holding the female protozoan macrogamete and fertilizes that macrogamete. This combining ofmale and female gametes is termed sexual reproduction and it results in the formationof a zygote (a fertilized 'egg'). A rigid wall forms around the fertilised zygoteand, once this wall is firm enough to withstand the harsh world outside of the intestinaltract, it erupts from the intestinal cell and gets shed into the faeces as an oocyst. Oocysts are shed in large numbers when lots of protozoan replication is occurringand clinical signs are evident. They are also shed, albeit in much smaller numbers, when a coccidia carrier state develops and no symptoms are seen (see next paragraphs).
From the time of ingestion of the infectious oocysts or intermediate host tissue cysts, it takes 4-11 daysfor the first oocysts to appear in the faeces of the definitive host animal (depending on the coccidianspecies). This period is termed the prepatent period.
4. Development of a non-clinical coccidial carrier state (the role of the host immune system in the appearance of clinical symptoms):
The cycle of organism replication and intestinal cell-death is only stopped or slowedwhen the definitive host animal's immune system responds to the invasion, killing all the freely-migrating merozoites it finds. The only way that the protozoan organisms are able to avoid this immune system attack is by hiding out inside of the animal cells in their clustered forms. These organisms alter their activityand reproductive processes, only replicating very slowly and only to the point whereby there are manyprotozoans in the cluster, but not enough to destroy the protective host cell shielding them.The replicating merozoites don't want to be exposed to the immune system attack that would occur were they to destroy their protective host cell. These slowly dividing protozoans, lying dormant within the intestinal cells of the definitive host animal, can betermed bradyzoites (brady means slow).
Author's note: as mentioned in the intermediate host section of section 4a(tissue cyst section), many coccidians are only able to undergo a fixed number of asexual replication repetitions. When the definitive host's immune system response kicks in, instead of these final-replication-stage merozoites erupting from their intestinal cells and differentiating into their sexual replication forms (ready to produce oocysts), many will choose to remain protected in their intestinal cells in dormant bradyzoite forms. After all, there is no point coming out of the intestinal cells only to be killed by a waiting immune system. These organisms bide their time, waiting for a 'break in the immune system' to occur, whereupon they will reactivate andcome out of the intestinal cells (when it is safe to do so) and continue the process of oocyst formation.
Bradyzoite protozoan organism clusters are not killed by antibiotics because they hide inside of cells where the antibiotics can't reach them, and, despite the fact that these organisms are forced to massively suppress their rapid-growth-rates in response to the immune system's presence, they are not actually killed by the immune systemeither. Consequently, in an animal with a healthy immune system, a situation will develop wherebythe animal is infected with intestinal coccidiosis, but, due to its strong immune system defenses, will have very little protozoan organism replication, very little intestinal cell injuryand, consequently, no clinical signs. This is the coccidia carrier state. Some oocysts will still manage to evade the immune system's attack and get produced from time to time, but the numbers will be low compared to the numbers of oocysts produced during clinical infection. These oocysts will infect the environment and sometimes get detected on fecal floatation tests (see section 5 - coccidial diagnostics), but the animal will not show any signs of disease.
If the immune system's defenses start to fail (e.g. through immune suppressant diseases, through immunosuppressant drugs, through stress, concurrent diseases etc.), the bradyzoites in the cells of the intestine will reactivate in the presence of this lax defense system. They will revert to a more aggressive form of asexual and sexual replication: replicating rapidly, causing lots of cell invasion and intestinal cell damageand creating clinical signs of infection. During this period of reactivation, lotsof gametes will be formed from final-stage merozoites and plenty of infectious coccidial oocysts will appear in the faeces. It is for this reason that animals with long term or repeated bouts of clinical, symptomatic coccidiosis need to be examined foran underlying immune system or stress-related problem. Normal animals should not keepgetting clinical coccidiosis - their immune systems should be able to hold coccidia in check!
Author's note: One of the short-falls of fecal flotation (see section 5), the major diagnostic test for coccidiosis, is the issue of 'intermittent coccidia shedders'. These are animalsaffected with coccidia that only release oocysts into their feces every few days or weeks, thereby making coccidia carriers very difficult to detect on a routine faecal float. It is thought that this intermittent shedding occurs as a result of natural ebbs and flows in the animal's immunity. The immune system regularly and periodically (due to physiological stresses, rhythmic hormone shifts etc.)releases some of its control over the coccidia for a brief period of time, long enough for some of thecoccidia to reactivate and make and shed oocysts, but not long enough for clinical signs to appear.
4b) Coccidia symptoms (coccidiosis) - the effects on the intestinal tract.
1. The roles of a normal gastrointestinal tract: 4) colonic motility and absorption of water:
The gastrointestinal tract (stomach, small intestine, colon and rectum) of the dog and cat is lined by a thin layer of cells termed an epithelium (i.e. the intestinal and colonic epithelium) or a mucosa. These cells have many important roles including:
1) secretion of mucus: Certain cells of the intestinal tract epithelium (especially the colon)secrete a protective mucus which is designed to protect the intestinal cells from the abrasive effects offood and feces passing along the tract and from the erosive effects of bacterial toxins and digestiveenzymes. The mucous also captures infectious organisms (bacteria, viruses and protozoa) that enter the intestinal tract and facilitates their expulsion with the faeces. The mucusproduced also lubricates everything, making the droppings slide along the intestinal tract easily. Intestinal mucus production will increase radically in the presence of intestinal irritants and chemicals, including infectious diseases like coccidiosis.
2) forming a cell barrier: The epithelial cells of the alimentary canal form a physical barrier, preventing bacteria andviruses and protozoans within the gastrointestinal tract from entering the deeper intestinaltissues, bloodstream and other organs.
3) gastric motility and expulsion of contaminants: The gastrointestinal tractis always moving, through the action of powerful muscles located within the walls of the stomach, small intestine and colon. Gastrointestinal motility allows bacterial toxins, invadingorganisms and excessive bacterial populations to be removed from the intestinal tract, so that they cannot do as much damage. In the face of invading organisms and toxins, intestinal motility is often increased on purpose in an attempt to expel the invaders from the intestine: this results in diarrhoea. When the intestine is unable to move (is paralyzed), as a result of certain diseasesand drugs, bacterial populations in the bowel can multiply to vast numbers, resultingin intestinal injury and sickness.
The colon has a special role in the absorptionof water from the intestines - this helps to keep the body hydrated. The colon actually uses some of itsmotility in a retrograde (backwards) fashion in an attempt to keep faeces inside the colon for just that bit longer so that water absorption can be optimised. Diseases that reducesegmental colon motility can stop the colon from retaining feces and absorbing the water - watery diarrhea results. 5) transfer of nutrients into the blood:
The intestinal tract (especially the small intestine)is the region of the body where nutrients (fats, proteins, sugars, minerals and vitamins) from the foodare absorbed. Severe intestinal damage and diarrhea (which makes food move throughthe tract too quickly for it to be absorbed) can result in malnutrition, weight lossand mineral and vitamin deficiencies. The cells lining the intestinal tract (the epithelial cells)have a huge role in nutrient uptake - certain chemical secretions and 'gate-ways'present on the surfaces of these cells are needed in order for some nutrients to get absorbed. 6) absorption of water:
The colon (end of the intestinal tract) absorbsmost of the water that enters the intestinal tract (both through drinkingand through digestive fluid secretions). If the colon is unable to perform this vitalrole, severe, life-threatening diarrhoea and dehydration will result. 7) other roles:
The intestinal epithelial cells have many other vital roles. They secrete hormones and enzymes which play an essential role in the control of food digestion and intestinal motility. They produce certain digestive enzymes and stomach acids; they initiate nerve impulses that tell the animal when it is full; they secrete hormones that control the intricacies of pancreatic cell and bile duct secretions and they secrete hormones that control the intricacies of intestinal motion. 2. Which coccidia cause intestinal symptoms in dogs and cats?
The pathogenicity of the coccidia (the ability of the coccidian species to cause disease symptoms)depends on many factors including:1) the number of oocysts eaten
- large numbers of oocysts ingested at any one time can cause rapid-onset, severe disease. 2) how rapidly the organisms divide
- the faster the coccidian organisms divide asexually, the quicker the host cells are destroyed and more cells become invaded. 3) the size of the replicating cysts (tachyzoites)
- larger cysts release greater numbers of infectious merozoites into the intestine to invade more cells and create more damage. 4) the number of replication cycles needed
- coccidia with higher numbers of tachyzoite replication cyclesare able to invade more cells and create more damage before they are forced to become dormantin their bradyzoite forms or convert into sexual, oocyst-making forms. Coccidia with higher replication cycles may, however, experience a greater delay before the infectious oocysts will appear in the faeces. 5) invasiveness elsewhere
- some coccidia species will leave the intestine of the definitive hostand invade and replicate within tissues elsewhere in the host's body. This may result in nasty disease symptoms, similar to those seen in some intermediate hosts. Generally, this phenomenon only occurs in very immune suppressed animals and in certain, specific hostanimal species (e.g. echidnas). Isospora
is responsible for most of the intestinal symptoms of coccidiosis seen in the dog and cat.Hammondia
do not cause intestinal disease in dogs and cats, despite replicating in their intestines, and require no treatment. Sarcocystis
, likewise, does not causeintestinal disease symptoms in the dog and cat, however, some species of Sarcocystis
may invade other organs of the dog and cat intermediate host, (e.g. the brain) and produce life-threatening symptoms relative to these areas (see section 4f). 3. The initial stages and symptoms of coccidia infection.
When the cells of the intestinal and colonic epithelium become heavily infected with replicating coccidiaorganisms (destructive, space-occupying tachyzoites and bradyzoites), they become damaged and start to degenerate. Ulceration of the lining of the gastrointestinal tract (especially the smallintestine) results, leading to severe irritation and inflammation of the affected regions.This severe inflammation and irritation of the bowel lining causes the intestines to spasm and the affected animal to display symptoms of abdominal pain. If the colon (large intestine) is alsoinfected, the animal will display symptoms of colitis - flatulence, straining to defecateand increased the frequency of defecation. Typically, animals with colitis (inflammationof the colon) will repeatedly and frequently squat as if needing to do a poo. Owners often think that their pet's repeated posturing and straining to defecate (termed tenesmus) is a sign that their pet has constipation. Animals with coccidia-induced colitis are not constipated, however: they just have a very irritated colon which keeps 'tellingthem' that they need to go to the toilet. Some animals will even vocalize (cry, whine) when straining to defecate because of their colon pain - this increases owner suspicions that they are constipated.Image 1:
A dog with colitis straining to defecate.Images 2:
Blood and mucus-filled faeces, typical of colitis.
In response to the infection and inflammation of the small intestine (and colon),large volumes of watery, protein-filled inflammatory fluids and mucous flood the intestinal tract. Mucus production, in particular, can be so great that large amounts of jelly-like slimeappear in the droppings and diarrhea of the animal (see the images above). If the amount of inflammatory fluid and mucous entering the intestinal tract is too much for the colon (the major water-absorbing part of the bowel) to reabsorb, then severe,watery diarrhea will result. This reabsorption of intestinal fluid and mucous secretionsby the colon will be even further impeded in puppies and kittens that develop severecoccidial colitis in addition to the usual small intestine infestations - inflammation and ulceration of the colon lining results in the colonnot being able to absorb water properly (even the water that these animals drinkmay not be absorbed). To add insult to injury, this coccidial diarrhea will oftenbe further exacerbated by increased intestinal motility: in an attempt to expel intestinal invaders out into the feces, the intestines will deliberately increasetheir motility. Unfortunately, this not only pushes out the invading bugs, it also pushes out vast amounts of much needed food and fluid (water), thereby making the reabsorption of water inefficient and the diarrhoea worse. Ultimately, what happensis that the severe, watery diarrhea and the significant intestinal fluid losses(the leaking inflammatory fluids and the excessive mucous production) result in anoverall loss of fluids from the animal's body. The young animal can become severely dehydratedand very sick.
If ulceration of the lower small intestine and colon is significant (i.e. if lots of cells are being destroyed asa result of coccidiosis), then bleeding can occur. Significant bleeding will become visiblein the stools: it can range from a few small spots or flecks of blood, in amongstthe mucus and slime, right through to large amounts of bright, red blood. Sometimes intestinal bleeding can be so severe that no feces are appreciated - the animal looks as though it is defecating straight blood! This is, as you can imagine, very frightening for owners to see!
Animals with significant coccidiosis and small intestinal damage may display signs ofvomiting, in addition to their diarrhoea. Vomiting further exacerbates the animal's degreeof dehydration because, not only is the animal losing fluid through its liquid stools,it is also unable to keep any of the water that it drinks down in its intestines.
If ulceration and inflammation of the intestines and colon is significant, affectedanimals may also display signs of fever. Symptoms include: a hot mouth and nose, red gums, panting, drinking excessivelyand seeking out of cool areas to lie down. The affected animal may also show signs of lethargy and sleepiness during these early stages, both as a result of fever and as a result ofdehydration. NOTE
- fever is not a typical or reliable presentation of animals with coccidiosis (don't rely on itas a clinical finding). Author's note:
the vast majority of kittens and puppies seen for coccidiosis are not thatsevere or sick-looking. They are generally quite bright and alert and playful and they are normallystill eating and drinking very well at home. They may have signs of abdominal pain and theyare often producing vile-smelling, watery to custard-consistency yellow or tan-coloureddroppings containing lots of slime (jelly), with or without the presence of blood. Vomitingmay or may not be seen. These animals often do not need any in-hospital care and they often do fine after some medical treatment at home.
4. Complications of coccidial infection: severe dehydration and protein and electrolyte losses.
If the diarrhoea (+/- vomiting) is severe and is left untreated or inadequately treated, the affected animal can become severely dehydrated. Please remember, dear reader, that these animals will often look as though they are drinking heaps of water. The problem is thatthese animals are physically unable to drink enough water to make up for their diarrhoealosses - much of the water that they do drink will remain unabsorbed and go straight outthe back end! Animals not given appropriate supportive veterinary care (includingintravenous fluid support) can become so dehydrated that they go into shock. Their mouths, feet and ears will become cold to the touch. They will become weak and wobbly on their legsand may progress to the point of being very non-responsive and unable to walk or lift their heads (cold, floppy puppies and kittens). Their gums will seem pale and dry and cold and their temperaturesmay become subnormal (under 37.5 degrees C). Their heart rates will be very fast. Left untreated beyond this point, severe dehydration and shock will cause failure of the liver and kidneys(organ shutdown) and, eventually, decompensation and slowing of the heart rate (a low heart rate in avery shocky animal is a very bad prognostic sign) and death.
In addition to dehydration (water-loss), severe inflammation of the gut and the loss of intestinalsecretions through the diarrhea can result in a severe loss of blood proteins and body salts, otherwise known as electrolytes (potassium, sodium and chloride), into the bowel. Severe reductions in blood salts(potassium in particular) can produce symptoms of profound weakness and unsteadiness onthe legs and may also interfere with the animal's heart rhythm and brain activity (strange neurological symptoms and even seizures can result). Severe loss of blood proteins can occur as a result of both inflammatory fluid loss and whole blood lossinto the bowel. Severely low blood protein levels can result in the fluid within the blood vessels (plasma) leaking out of the blood vessels into other areas of the body. This will result in a dropin the animal's blood pressure (worsening shock) and, depending on where it settles out, the leakingfluid can interfere with the function of other organs (e.g. fluid in the lungs can cause difficulty breathing,fluid in the brain can result in brain dysfunction, seizures and coma).
5. Complications of coccidial infection: if colonic bleeding is severe.
Animals (especially young puppies and kittens) can bleed enough from coccidial induced intestinal ulceration that they become severely anaemic. Anemia is a condition whereby there is not enoughred blood cells in the animal's circulation to carry oxygen adequately around its body. These animals develop a rapid heart rate and respiratory rate and very pale to white gumcolor (see images below). Left untreated, these animals can suffer severe tissue and organ damage(e.g. brain damage and renal failure) from a lack of circulating oxygen and may eventually becomecomatose and die. Lots of blood in the faeces, particularly in a young kitten or pup,should be a big concern to owners and urgent veterinary attention is recommended.
Image 1: This is a dog with very pale gum color. Ignore the black/brown pigmentationon the animal's gums and look at the spaces between the black spots - see how paleand white they are? These regions should be a bright salmon pink colour. This is adog with severe anaemia.
Image 2: This is the feces (believe it or not) from a dog with very severe colitis. The diarrhoea is almost frank blood, aside from the whitish coloured flecks visible throughoutit (the white flecks are mucus chunks produced by the irritated colon).
6. Complications of coccidial infection: secondary bacterial infections.
The coccidial infection strips away the protective epithelial cells lining the intestines and colon, resulting in large ulcers that expose the more-fragile, underlying intestinal tissues.These bleeding ulcers provide a perfect site for the nasty bacteria of the gut (including true nasties like Salmonella, E.coli and Clostridium) tocolonize and the large volumes of inflammatory proteins and secretions within the damaged bowel provide nutrition for the bacteria, allowing their populations to grow quickly. These bacteria are given plenty of opportunity to multiply and increasein number because the damage to the intestine reduces and/or alters its motility, thereby allowing the bacteria to remain and grow in one site rather thanbeing moved on by normal gut motion. Once the bacterial population reaches significant numbers, these secondary bacterial infectionscan start to cause bowel problems of their own. Bacteria release nasty toxins and bowel-damagingchemicals that further injure the intestinal lining, worsen the diarrhea symptoms andmake the animal feel miserable, sick and feverish. Animals with severe secondary bacterialinfection may go off their food and can appear very unwell.
Author's note: These secondary bacteria can often be detected on fecal culture and seen on rectal or faecal swabs. It can be difficult for the vet, when confronted bycoccidia as well as intestinal bacterial pathogens, to know which organismstarted the problem off (which came first). The bacteria could be the primary issueand the coccidia just a secondary organism taking advantage of the bowel injuries to replicate or,alternatively, the coccidia could be primary problem and the bacteria the secondary opportunists.
If the secondary bacterial infection progresses, eventually bacterial organisms and/or their bacterial toxins may enter the bloodstream, resulting in severe illness, septic shock and the potential for multiple organ infection/damage and even organ failure. These 'septic' animals are very weak and lethargic and are often febrile(feverish). They are usually inappetent and may have even progressed to the pointof frequent, projectile vomiting. Their feet and faces will feel hot to touch and they will pant a lot. If they are not anemic, their gums will often take on a horrendous dark, brick-red to purplish colour: a result of fever, dehydration and septic shock(in septic shock, the animal's peripheral blood vessels dilate inappropriately, droppingthe animal's blood pressure severely). Death will often be the result.
Circulating bacteria can become deposited into the small capillaries of the joints, eyes, heartand kidneys, leading to infection of theses places. Septic arthritis (bacteria in the joints), in particular, is a well-known complication of parvo virus and many other nastyulcerative intestinal diseases in puppies and kittens.
Image - these are the brick-red coloured gums of a dog with severe septicemia. This particular animalhad pneumonia, but could easily have had severe bacterial colitis or enteritis.
7. Complications of coccidial infection: intussusception and rectal prolapse:
Small intestines and colons infected with coccidia can become very thickened as aresult of severe ulceration and inflammation of the intestinal wall. The motility of the infected bowel is often abnormal: some parts of the intestine may be excessively hypermotile (moving too much in response to infection - trying to clear the infection) and other parts may be excessively hypomotile (not moving enough at all as a result of damage and inflammation). This abnormal intestinal motility can occasionally result in the coccidia-affected intestine or colon telescoping into itself, causing a complication caused an intussusception.Sections of bowel trapped inside of other sections of bowel can result in severe rotting and damage of the intestine if left untreated. Correction of this complication requires surgery: surgery that is likely to be done on an already malnourished, possibly septic, and compromised animal.
In addition to intussusception, animals with severe colitis (including coccidiosis)can sometimes develop a complication called a rectal prolapse. This is where the rectumitself comes out of the bottom, like a large pink sausage. It occurs when animalsstrain and strain to defecate as a result of colonic irritation - eventually this straining can result in the whole rectum coming out. Left untreated, the exposed rectumcan rot and die off, resulting in severe illness and, potentially, the death of the pet.Surgery may be required to replace the rectum, should manual techniques fail to return itto its correct place.
Image 1: This is an image of a young cat with a rectal prolapse.
Image 2: This is a close up of the prolapse, showing that it is coming out of the anus.
Image 3: This is an end-on view of the prolapse, showing the tunnel which is thecentral canal of the colon.
8. Complications of coccidial infection: long-term, chronic infection and weight-loss (fading kittens and puppies):
The small intestine (the main site of coccidia invasion) is the main site of nutrient absorption and uptake in the animal body. Some kittens and puppies affected with coccidiosis do not become acutely and severely ill from dehydration and secondary bacterial infections,as described above. Instead, some of these kittens and puppies develop a lower-grade, chronic, longer-term coccidial infestation of their small intestines which, although it does not rapidly kill them, causes enough small intestinal damage that they are unable to absorb their food properly. Unable to absorb their food properly, these chronically affected kittens and puppies will become malnourished and will steadily lose weight and condition.They will become thin and underweight, with coarse, starey hair-coats and bony frames. Their faeces,although not watery, will often be loose and soft with whole, undigested food particlesappearing in the droppings. These animals will often be ravenous (starving hungry) andwill eat everything they can get hold of and yet, despite this good appetite, they will keep losing weight. Many of these creatures will die as a result of chronic wasting (fading kittens and puppies) and as a result of increased susceptibility to other infectious diseases (malnutrition reduces their immune system defenses to all diseases).
4c) Chronic, recurrent coccidia infections - coccidia that keeps returning.
In the vast majority of cases of non-complicated coccidiosis, the antiprotozoal drugs and other therapeutics prescribed by the veterinarian will have their desired effect: the symptoms of coccidial infestation will abate and the puppy or kitten (or older pet) will be fine. In a smaller proportion of patients, however, the symptoms of coccidiosis will persist, despitethe treatment given. These symptoms may either completely refuse to go away at all, even during theperiod of medical treatment, or they may go away for a period of time during and after treatment (during which time the animal will appear to defecate normally), only to reappear later, days to weeks to months down the track.
So what is happening here?
Well, there are many reasons why the symptoms of coccidiosis may persist or keep on recurringwithin the one animal. These are as follows:
1) The primary disease is not coccidiosis (i.e. the symptoms of enteritis and colitis being treated are not being caused by coccidial organisms):
There are many disease conditions that can closely mimic the intestinal symptoms of coccidiosis.The list is large and includes a wide range of parasitic diseases, bacterial diseases, viral diseases, inflammatory bowel conditions, structural intestinal and colonic diseases (bowel cancers etc.), constipation disorders, intestinal accidents (e.g. intussusception, volvulus)and intestinal foreign bodies. Some of these diseases are very simple to diagnose and differentiate from each other using basic physical examination techniques (e.g. rectal examination can quickly diagnose constipation - the presence ofhard, impacted faeces in the rectum and colon) and/or basic diagnostic testing methods (e.g.whipworm infestation can be easily diagnosed on fecal flotation if whipworm eggs are found), however, some of the causes of intestinal disease symptoms can be very difficult to diagnose by simple testingmethods alone. They need more advanced diagnostics. Making the task even more difficult for veterinary diagnosticians is the fact thatsome of these intestinal conditions may even occur concurrently with other intestinal diseases,such as coccidia, making the primary cause of disease symptoms tricky to determine.Vets that fail to detect the 'more difficult to diagnose' intestinal diseases or fail torecognize the presence of another, concurrent, primary disease condition, may easily misdiagnose an animal as having coccidiosis, particularly if a couple of coccidialoocysts get seen in a fecal float.
As mentioned previously, coccidian oocysts can often be found in the faeces of normal dogsand cats whose immune systems have never been 100% able to clear the parasite infection (i.e. the coccidia carrier state). These non-symptomatic carrier animals can become affected by another non-coccidian intestinal disease (e.g. inflammatory bowel disease (IBD), intestinal bacterial infections like Clostridium), develop symptoms of gastroenteritisor colitis and be misdiagnosed as coccidiosis because the vet finds coccidia oocysts intheir faeces (see section 5). The fact is, these coccidia carrier animals wouldhave had the coccidial cysts in their feces even in the absence of any intestinal signs. Should the vet misdiagnose the animal as having coccidiosis and treat for that disease, it is very likely that the intestinal symptoms will persist. This is because the primary intestinal disease problem (e.g. IBD, bacterial infection etc.) has not been diagnosed or treated. As a result, the vet and owner will think that the coccidiainfection is persistent, when, in fact, the disease symptoms seen are due to something else entirely.
Even more confusing for vet and owner: because coccidia is not cleared from the intestine by most anticoccidial medicinesand because it is an organism that tends to replicate opportunistically in the presence of concurrent intestinal disease (i.e. it takes full advantage of a sick intestine), the next time that the patient appears to the vet with those same intestinal symptoms,it is highly probable that coccidial oocysts will still be discovered in the faeces. This finding will serve to further convince the vet that the coccidia is the causeof the problem, even when it isn't. The vet might even decide to trial a differentanti-coccidial medication this time around, however, no improvement will be seen. The intestinal problem and symptoms will persist, regardless of coccidial treatment applied, until the primary, non-coccidial, intestinal disease is found and sorted. Further work-up, including: rectal smears, fecal ELISA tests, abdominal radiographs (x-rays), abdominal ultrasound, colonoscopy and colon biopsy may be needed to find thetrue cause of the symptoms.
2) The coccidial organism(s) diagnosed on fecal flotation are not the right kind:
As mentioned previously, most coccidia species are host specific when it comes to the types ofanimals whose intestines and colons they will infect. An oocyst belonging to a cat- or pig-adaptedcoccidian organism will not hatch in a dog intestine and nor will it replicate to cause symptoms of intestinal disease in a dog. Instead, should a dog consume the droppingsof a coccidia-infected non-canine species of animal (e.g. a dog that regularly consumes pig poo or cat droppings),any oocysts ingested will pass through the dog's intestines unhatched and come out, unharmed,in the stools of that dog. A veterinarian who subsequently performs a fecal flotation on that dog's droppings may misdiagnose that dog as having coccidiosis shouldhe or she fail to recognize that the coccidial oocysts seen are not of the dog-infectious variety.Similar to the situation described in point 1 of this section, symptoms of intestinal diseaseseen in this kind of patient will not respond to coccidiosis treatment because the coccidianorganisms found in the stools are not the cause of the issue. The animal will continue to show abnormal intestinal symptoms (the coccidiosis will seem to 'persist') until the trueintestinal issue is diagnosed.
See image: The coccidian oocyst on the left is Isospora felis, a known pathogen of the cat. Finding lotsof these in a cat's dropping may be significant. The coccidian organism on the right is not Isospora: it could be a non-pathogenic cat-adapted organism such as Hammondia orit could be a fecally-ingested oocyst from a non-cat species of animal that is just passingthrough. Even though they look impressive, finding lots of these in a cat's droppings may not be significant at all.
In the case of a dog or cat that regularly consumes the faeces of other animals,frequent symptoms of gastrointestinal upset and intermittent enteritis and colitis are more likely to be causedby the animal's dietary indiscretions (eating garbage and rotten faeces) and/or nastybacteria present in the poo (Salmonella, E. coli, Clostridium, Campylobacter).Diagnosing atypical coccidial species in the patient's droppings will at least givethe vet a vital clue that this animal is probably wandering and that it is scroungingfor droppings and probably other rotten foodstuffs and carcasses as well.
Fecal oocyst culture - see section 5c - is required in order to diagnose the species of coccidian oocyst present in the stools. Rectal smears and fecal bacterial culture can be used to diagnose bacterial pathogens that might be the cause of intestinal symptoms following fecal consumption.
3) You are dealing with a true coccidial infestation (i.e. not a misdiagnosis) that keeps on re-activating:
The re-emergence and re-activation of latent (dormant) coccidia infestations is the most common reason for prolonged (chronic) or recurrent symptomatic coccidian infections.
As mentioned previously, coccidian infestations do not tend to be completely cleared from the body by the host's immune system. In most animals, the coccidian organismsand the host's immune system establish a balance whereby the coccidia are unable to replicate rapidly andcause any disease signs in the host, but the host's immune system is unable to clear them from the body. This balance between host immune system and parasite can last for months to years without the dormant, latent coccidial organisms reactivating orcausing any clinical disease symptoms.
If the host immune system fails to maintain this balance, however, the coccidians can re-emerge, replicate quickly and produce disease symptoms in the host. Should the immune system recover quickly(e.g. the immune system was only 'down' for a short while because of a brief period of intense stress), then that animal will recover and the coccidia will be forced back into a dormant statewith no more clinical symptoms. If, however, the animal's immune system is rendered ineffective for a long period of time (e.g. due to immune disease; long-term immune suppressant drugs; chronic, long-term stress situations), then the coccidia will persist and become a longer-term clinical problem. These animals will persistently displaysymptoms of enteritis and shed coccidia in their faeces. The only time that symptoms may appear to abate in these animals is when those animals are being treated with antimicrobial drugs: the affected animals will seem to recover from the infection, only to relapse as soon as the drugs are discontinued. This relapse occurs because most coccidial antimicrobial drugs are unable to completely clear coccidia from theintestines and because treated animals often soon get new infestations from a contaminatedenvironment.
Recurrent and persistent symptomatic coccidial infections can occur in animals that are exposed to a lot of stress - e.g. poor husbandry, conditions of extreme heat and cold, poor or imbalanced nutrition, concurrent illnesses, lactation, weaning or pregnancy, transportation, overcrowding, altered environment, re-homing, owner absence etc.They can also occur in animals that have immunosuppressive diseases (e.g. immune mediated disease, diabetes mellitus, Hyperadrenocorticism (Cushings disease), AIDS, FIV (feline AIDS), FeLV (feline leukaemia virus), cancer, leukemia or congenital (genetic) immune disorders) or that are on immune suppressive medications (e.g. corticosteroids, chemotherapy drugs).
The work-up (diagnostic steps) of chronic and/or recurring coccidial infestations involves two vital steps:
A) rule out other causes of intestinal disease first: remember points 1 and 2 - symptoms of intestinal disease can be caused by a number of other intestinal disorders, commonly misdiagnosed as coccidiosis
B) if coccidia is the only identifiable cause of the intestinal symptoms seen, determine whetheror not there is an underlying source of stress and/or immune suppression and remove it (if possible). Failure to remove any underlying immune suppression (including stress) will result inthe coccidia persisting and disease symptoms constantly reappearing.
4d) Coccidia diagnosed in adult dogs and cats - is it significant?
Significance of adult animals with coccidia symptoms:
The main time we vets tend to encounter clinical symptoms of disease caused by coccidian organismsis in very young kittens and puppies, under 6 months of age (usually much younger - around 1-4 months of age). Young animals' immune systems are naive (have not been previously exposedto the parasite) and their immune defense responses take time to adapt to the coccidialinfection and to suppress it. The time taken for the juvenile immune system to adapt to and respond to the new parasite infestation allows the coccidial parasites to get a head start with their replication and cell destruction - this is the reason why young animals are most likely to show clinical symptoms of coccidial disease. Although coccidial oocysts can often be found in the faeces of older animals, it is much more uncommon for adult animals toactually show clinical symptoms of coccidial infection. This is because the immune systemof an adult animal has often 'seen' (been exposed to) the parasite before and, thus, is quick to dealwith it before the organism has a chance to replicate and cause disease symptoms. For the most part, coccidia ova (oocysts) found in the faeces of older animals, particularly animals showing no symptoms of disease, are not of much clinical significance at all.
An older animal (6-12 months or more) that has never before been exposed to coccidian organismsmay develop clinical symptoms of coccidiosis if it ingests the organisms from its environment.Similar to the situation seen in young pups and kittens, these clinical disease signs wouldoccur because of the naive state of this adult animal's immune system and the timetaken for that immune system response to adapt and develop and respond appropriately. It would be expected that such an animal would display mild symptoms ofcoccidiosis (probably not as severe as the disease seen in the very young) and shed lots of coccidial oocysts into its feces until its immune system kicked in and suppressed the infection, thereby resolving the clinical signs.
Although the situation described in the paragraph above (coccidia infection ofan immunologically naive adult animal) can indeed occur, it must be mentionedthat such an occurrence is relatively rare. Coccidia is such a ubiquitous, wide-spreadparasite that it is uncommon for most dogs and cats to get to adulthood without havingever encountered it before. Consequently, I would always interpret a diagnosis of clinical coccidiosis in an adult animal with some degree of caution. Clinical coccidiosis is really not that common in adult animals, particularly animals that have previously had thedisease in kitten-hood and puppy-hood, and, although I would certainlytreat for the condition if I found it on a faecal float, as a veterinarian I would alwayskeep at the back of my mind the possibility that this animal could just be a coccidia carrier (alwaysasymptomatically shedding oocysts) with some other colonic or intestinal disease causing the symptomsseen. Alternatively, if that adult animal did indeed seem to have a true case of coccidiosis (heaps of oocysts in the poo, supportive clinical signs etc.), I wouldalways consider it a possibility that this animal could have an underlying stressor orsome other immune suppressive disorder making the parasite appear and cause disease. My suspicion of an underlying immunosuppressive process would be increased further should this animal go on to show repeated bouts of clinical, symptomatic coccidiosis.
Significance of adult animals as coccidia carriers:
As mentioned previously, adult animals with no clinical symptoms of coccidiosis(the animals are termed carriers) will often be found to have low numbers of coccidial oocysts in their faeces on a faecal float. Although this subclinical coccidia infestation is unlikely to be of clinical disease significance to that carrier animal (we don't even recommendmedical treatment for it), it must be re-iterated that this non-clinical shedding of oocysts intothe faeces is of significant risk to young, susceptible animals. Young animals thatconsume these faeces will ingest the oocysts and are likely to show significant symptoms of disease compared to their adult carrier counterparts.
In order to reduce the spread of coccidiosis to young, susceptible, immunologicallynaive animals, adult carrier animals should not be housed in the same pens or runs as underage animals (under 6 months). This is particularly important if coccidiais to be ever controlled in large, multiple dog or cat facilities such as boarding kennels, catteries, breeding facilities, pet-shops and vet clinics.
Carrier animals also pose a risk to immunosuppressed older or younger animals. Animalson chemotherapy or that have immune system disorders or high, long-term stress levelsmay develop clinical signs of coccidiosis, should they consume the organisms froma carrier animal's stools.
Author's note: The clinical significance of any coccidial infestation, be it in older animals or younger animals, must take into account the species of coccidian oocysts discovered. Hammondia, Besnoitia and Sarcocystis oocysts are generally of little clinical significance to the definitive host - these organismsdo not usually cause significant intestinal disease or intestinal signs in the dog or cat. Finding the oocysts of non-dog and non-cat species of coccidian organisms in a dog or cat's faecestells us that the dog or cat has been eating the infected poo of other animals, but that coccidiosis is not the cause of any intestinal upsets seen (atypical coccidia oocysts simply move through the intestines of the dog and cat without hatching or replicating).
4e) Can animals be infected with coccidia and show little or no signs of disease?
This has pretty much been discussed in sections 4a and 4d, but I will summarize it here.
Animals that have previously recovered from coccidial infestation (regardless of whether they receivedantimicrobial medications or not) will often go on to develop a coccidia carrierstate. A carrier state means that these animals are still infested with the organismand still intermittently shed the infectious organism oocysts in their faeces, but theyshow no clinical signs of disease. The carrier state develops because the animal's immune systemresponse to the organism is enough to suppress the organisms' rapid growth and replicationin the gut, but not enough to clear the organisms completely from the body (the organism hides outinside of the animal's intestinal epithelial cells, thereby avoiding the immune system's destruction).
Carrier animals pose a huge risk to young, susceptible animals and immunosuppressed orstressed animals because they shed infectious oocysts into their faeces, which might beconsumed by such animals. Carriers are a massive problem in multiple-dog or multiple-cat facilitiesbecause they are difficult to identify and isolate from the rest of the population and because they constantly shed the resistant oocysts into the environment. Treatingcoccidia infected puppies and kittens costs such businesses lots of money, as does the staffingand chemical control (disinfectant) methods needed to combat and manage the disease.
4f) Sarcocystis neurona - equine protozoal encephalomyelitis.
Sarcocystis neurona is a parasite of the alimentary tract (gastrointestinal tract)of opossums that can invade the tissues and organs of a range of intermediate host species,including cats, horses and a number of wild animal species. When the infectious, fecally-shed oocysts are ingested by a horse or cat or wild animal, the protozoan tachyzoitesinvade and replicate rapidly within the brain cells of that animal, producing severe, often fatal,neurological disease symptoms. The infected animal may display a range of different, progressive neurological signs including: wobbliness, incoordination, lameness, paralysis, muscle wasting and fecal and urinary constipation or incontinence.Seizures, coma and death may be the end result. The disease condition is well-known in American horse facilities, where it is termed equine protozoal encephalomyelitis.The disease is only just being recognised in cats and is not so well-known in this species.
Diagnosis of the disease in horses is generally achieved by detecting an increased level of S. neurona antibodies in the cerebrospinal fluid (CSF) of the infected horse using Western Immunoblot techniques. PCR, specially designed for the amplification and detection of S. neurona DNA,can also be used to detect the organism in the CSF samples of cats and horses. Only a few specific, diagnostic laboratories are equipped to run these tests. If the patient happens todie from the disease, diagnosis can also be made postmortem by the histological examination ofthe animal's brain tissues - tachyzoite and bradyzoite cysts can be easily seen in infected braincells.
Treatment of S. neurona infection is not always successful, but attempted medical protocols in horses include:
Pyrimethamine at 0.1-0.25mg/kg once daily for 4-12 weeks in conjunction with 7.5-10mg/kgtwice daily trimethoprim sulfonamide antibiotics. Trimethoprim sulfonamide drugs access the brain tissues and CSF of animals quite well, making them very useful for protozoan brain diseases.
Ponazuril, a close relative of Toltrazuril, has also been trialled successfully in horseswith equine protozoal encephalomyelitis.
At the moment, management and treatment of the feline form of Sarcocystis neurona infectionhas to be extrapolated from equine drug dosing regimens. The disease is relativelynew in the cat medicine world and more study needs to be done on it before optimalmedical regimens are able to be recommended.
Author's note: Sarcocystis neurona has been known to cause severe neurologicaldisease symptoms in skunks and raccoons. These particular wild animal species are also known to be common carriers of rabies and distemper - viral diseases which also produce neurological symptoms. Rabies is a very dangerous disease to man and wild animals with neurological disease signs should never be approached. Don't make the mistake ofassuming that neurologically affected skunks and raccoons have S. neurona - always treat them as possible rabies carriers and keep well away! See our comprehensive rabies page for more on rabies disease, treatment and prevention.
This disease condition is most 'common' to the USA and places that have opossums. As far as I can tell, the condition is exotic to Australia - Australia has possums, but not thekinds of possums (opossums) that are encountered in the United States of America.
5. How is coccidiosis diagnosed? - Testing for coccidia.
Coccidiosis is routinely diagnosed using an in-house test called a faecal flotation test(also known as a Fecalyzer test or fecal float test). The aim of this simple test is to detectcoccidial oocysts (the infectious forms) in the stool samples of infected definitive hostanimals (e.g. dogs and cats). Other supportive diagnostic tests, including: fecal smears (wet preps), fecal oocyst cultures and intestinal biopsies, may also be required in order to make a diagnosis or add to a diagnosis of coccidiosis.
5a) Fecal flotation.
Faecal flotation is a test performed on the faeces of the clinically infected or carrier dog and cat, or any other animal species that is a definitive host for some species of intestinal coccidian. The test is performed in order to detect the presence of tell-tale coccidial oocysts in the faecesof the infected animal, thereby supporting a diagnosis of coccidiosis. The test can alsobe used to detect the fecally-shed oocysts of certain other parasitic protozoan organisms, including Toxoplasma, Cryptosporidium and Giardia, as well as the eggs of various parasitic worms, including whipworm, tapeworm, roundworm and hookworm.
What the fecal flotation test entails:
The fecal float test involves mixing the faecal sample in a suspension of sodium nitrate solution (Sheather's sugar solution or zinc sulfate solution may also be used) using a special apparatus called a Fecalyzer (or Fecalyser). The faeces (which are heavier than sodium nitrate and the other solutions) sink to the bottom of the solution and the parasitic worm eggs andprotozoan oocysts float to the top of the solution. These eggs and oocysts gatherunderneath a microscope coverslip (a small square of glass placed on the top layer of the solution), which is later (after 10 minutes) placed on a microscope slide (a large rectangle of glass) for examination under a microscope. The veterinarian then examines the slide under the microscope, looking for obvious worm eggs and protozoan oocysts.
The image on the right is of a fecal float that was performed on the feces of a kittenwith diarrhoea. The test revealed lots of coccidian organisms.
For an excellent step-by-step guide to in-house faecal flotation, see our greatfaecal float page. The page contains great step-by-step pictures and photo images of how the testis performed and what is seen under the microscope.
Collecting the faecal sample:
As a general rule, the best stool samples to perform a fecal floatation on are those thatare as fresh and recent as possible (i.e. straight out of the dog or cat). Ideally, fecesshould be tested within 30 minutes of leaving the animal. The reason we want an extra fresh stool sample is because the eggs of some parasites (e.g. parasite worms) hatch very rapidly, making these parasites impossible to diagnose on aged stool samples. A fresh sample makes these parasites easier to detect. In the case of coccidia, the oocysts do remain viable for long periods of time and may be detected even in older poo samples (samples 1-2 days old), however, as a general rule: it is always best to go for fresh samples.
The veterinarianwill often collect an extra-fresh stool sample from your dog or cat's anus during the consult, using a gloved finger, or collect samples of faeces that your dog or cat passes in the consultationroom or cat carrier. Alternatively, you, as the owner, can help your vet to get a diagnosis bybringing your pet's most recent stool sample (e.g. diarrhea sample) into the consultation for flotation.
Abnormal stool samples can even be dropped off at veterinary clinics for fecal flotation,if owners are concerned about parasites in their pets. Although it is always recommendedthat owner and pet go into the clinic for a proper consultation and examination,busy owners on their way to work or owners unable to get an appointment until latermay attempt to get a more rapid diagnosis by dropping off samples of slimy, blood-tinged or diarrhoeic faeces for testing.
Fecal samples should be submitted in small, preferably sterile, air-tight containers such as fecalsample jars or urine specimen pots, with the lid screwed tightly into place. Your vet can provide you with an appropriate specimen container to collect your pet's faeces into. At a pinch, you can use a new, ziplocked, sandwich bag for carrying faeces into the veterinary clinic - just make sure you double-bag the sample so that it does not leak. Do not use oldjam jars - even when cleaned, these contain fungi that can confuse the diagnosis.
Owners should always wear disposable gloves when collecting faeces. Faecal samples should be sent in to the vet as soon as they are collected, however, if the sample is collectedafter-hours, you can place the well-sealed sample in the fridge for safe storage. Chilling the sample slows the development and hatching of any eggs and keeps the samplefresh. Do not put samples in the freezer.
Pitfalls of faecal flotation - false negative results:
It is possible for an animal to truly have coccidiosis, but for tell-tale coccidial oocysts not to be found on a fecal floatation test. These test results are termed false negative testresults and they occur for several reasons:
1) Coccidial oocysts can be shed intermittently - many species of intestinal protozoa, includingIsospora, many other coccidians and Giardia, are well known for having a cyclic patternof protozoan replication and oocyst production. These organisms tend to release oocysts into the animal's faeces in waves. The consequence of this is that some stool samples will, therefore, contain loads of oocysts and others, from the same animal, will show almost none. Any vet, who happens to be unlucky enough to get the stool sample without organisms,will come up with a negative result (a false negative), when other stools samples from the same animal might be full of the tell-tale oocysts.
2) Some coccidia shed very low numbers of oocysts - it is possible for ananimal to have clinical signs of coccidiosis and to only be shedding low numbers ofcoccidial oocysts into the faeces. Oocyst numbers are not a reliable indicator of whether theanimal is clinically infected with the disease or not. Obviously, large numbers ofoocysts are most supportive of clinical infection, however, low numbers of oocystsmay be all that are seen in some animals as an indicator of active infection. Becausefecal floatation is performed using a human eye, it is possible for very small numbers of oocysts to be missed, if the vet isn't careful and observant, thereby resulting in a false negative result.
3) Lab error and veterinarian inexperience - fecal float examination is askill acquired with years of practice. It is possible for inexperienced practitionersnot to recognise parasitic oocysts and eggs and to make a false negative diagnosis.
4) Destruction of the parasitic eggs or oocysts by the fecal float solution - Some ofthe solutions used in the fecal flotation process (e.g. sodium nitrate and sometimes even Sheather's Sugar solution) can distort or destroy the larvae, eggs or oocysts in the fecal sample, resulting in false negative results. Sarcocystis, as one example, undergoes complete maturation andsporulation (matures to the infectious oocyst form) inside the dog or cat intestine - theinfectious oocyst that results generally breaks apart during fecal flotation procedures, resulting in false negative results. Zinc sulfate solution is thought to distortand destroy oocysts, eggs and larvae the least of any of the flotation mediums and isthe medium of choice for Giardia and heavy eggs like Whipworm. Sheather's sugar solutionis preferred for coccidia, but will distort Giardia beyond recognition.
5) Certain parasite eggs do not float very well - some parasite eggs (e.g. whipworm) do notfloat well on the typically-used fecal float solution: sodium nitrate. This can result infalse negative results (eggs that don't float can't be detected). Different floatation solutions (e.g. zinc sulfate) are required to diagnose them. Sodium nitrate is usually fine for diagnosis of coccidiosis (most coccidia float very well).
6) Some coccidial organisms produce severe enteric signs before the oocysts actually appear in the poo - it can take up to 11 days (15 in some species), from the time of infection, before fecally-shed oocysts will begin toappear in the host's faeces, in order to be detected by a fecal float. Within this time, severe clinicalsymptoms of coccidiosis can occur, without there being any oocysts visible in the droppings.
Pitfalls of faecal floatation - false positive results:
It is possible for coccidial oocysts to be found on fecal floatation and forthe animal not to actually have coccidiosis as the main disease process. These test results are termed false positive results and they occur for several reasons:
1) The animal is a non-clinical carrier - many animals, particularly adult animals, can develop a coccidia carrier state, whereby the organism is replicating slowly withinthe intestines and shedding oocysts into the faeces, but not actually causing any clinical symptoms. If these carrier animals develop symptoms attributable to intestinal or colonic disease, the vet is very likely to find coccidial oocysts on a faecal floatation test, without the coccidiaactually being the cause of disease! This will result in a false positive result.Treatment directed at the coccidia is unlikely to resolve the symptoms of disease because the primary intestinal disease has not been diagnosed or treated.
2) The animal has clinical coccidiosis due to an underlying immune disorder - this is kind of a true positive/false positive situation. Carrier animals with coccidiain their intestines can revert back to clinical signs of coccidiosis if their immunesystem control over the coccidia organisms in their bowels fails. This can occur dueto stress or many other congenital and acquired immune deficiencies. Althoughfinding coccidia oocysts in the faeces of these animals is proof of the coccidian infectious disease causing the symptoms (a true positive), it can also be considered to be a false positive, if the underlying immune disorder or stressor that is causing the coccidia to become a problem is not diagnosed and managed too.
3) The coccidia species is not the right species for that host - as mentionedpreviously, dogs and cats that eat the droppings of other animal species can consumeoocysts that will travel, unchanged, throughout the dog or cat's intestines and be shedinto their faeces. Vets that fail to recognise these oocysts as non-dog and non-cat speciesof oocysts will incorrectly diagnose these animals as positive for the disease.
4) Lab error and veterinarian inexperience - lots of weird objects can be found infecal floats: anything from pollen balls to fungal elements. It is possible for inexperienced vets to incorrectly identify these objects as parasite oocysts and to, therefore,determine the animal to be positive for the disease.
5b) Fecal smear (wet prep).
A fecal smear, otherwise known as a wet prep, can be a very useful, simple test for detectingcertain parasites in extra-fresh fecal samples and rectal swabs.
The test involves taking a very smallsample of feces or colonic-mucus and breaking these faeces/mucus-samples up on a microscope slide using some saline solution (0.9% NaCl - sodium chloride). A coverslip (a tiny square of glass) is placed on top of the faeces-saline mixture and the sample is examined under a microscope for parasites. That's it! How easy!
Wet preps are valuable, simple diagnostic tests that can be used to detect a whole range ofparasitic organisms not easily detected on a fecal float. Motile (swimming) organismssuch as Giardia and Trichomonas, that are normally destroyed by fecalfloat solutions, are not destroyed by saline and, thus, may be diagnosed on fecal smears. Similarly, many other oocysts and worm larvae, normally destroyed or severely distortedby fecal flotation mediums, are not distorted by saline and, thus, may be detected onfecal smears. Heavy eggs, such as whipworm eggs, may also be found on fecal smears becausefecal smears do not rely on floatation techniques.
Despite their good qualities and ease-of-use, fecal smears should not be consideredto be a complete replacement for fecal floatation techniques. Fecal smears come witha major drawback: because such a tiny sample of feces or mucus is used in this test, only highly-numerous organisms that are diffusely distributed throughout all of the faeces are likely to be detected. Fecal floatation is a much better technique for detectingsmaller populations of organisms because greater volumes of faeces can be floated.The two tests should be thought of as supportive of each other - each having their ownstrengths and weaknesses. The two tests should be used in conjunction with each otherin any animal with symptoms of intestinal disease.
5c) Fecal oocyst culture.
The species of coccidian oocyst found in a fecal sample is generally determined by observing theoocyst in its fully-matured state and counting the numbers of sporozoites (infectious protozoanorganisms) and the number of sporocysts (compartments holding the sporozoites)within that mature oocyst. Because it takes time for oocysts in the faeces to mature totheir infective forms (whereupon the species of coccidian is determinable), veterinariansand pathologists need to wait for a few days before the fecally-shed oocysts can be identified.
In order to enhance the chances of obtaining a definitive oocyst species diagnosis, the vet or pathologist usually performs a fecal oocyst culture: placing the oocyst-infected faeces into optimum conditions of temperature and moisture so that the oocysts have the best chance possible of maturing into their infective, identifiable stages. To perform an oocystculture, oocyst containing faeces (as determined on a fecal float) are placed into a Petri dish containing a solution of 1% potassium dichromate. Only a shallow suspension of feces and potassium dichromate is placed into the dish because the organisms need the oxygen, diffusing into the suspension, to grow. After 2-4 days of incubating at room temperature, most of the coccidial organisms in the culture sample will have sporulated (matured) and should be identifiableon a repeat fecal float.
5d) Colonic or intestinal biopsies.
Taking biopsies of the small and large bowel and sending these biopsy samples off to a laboratory for histological assessment (assessment of the intestinal cells, inflammatory cells and the overall intestinal tissue architecture/structure) is not commonly done just for the purposes of diagnosing coccidiosis. Because it is an invasive procedure, intestinal biopsy is normally only performed when fecal flotation and other less-invasive methods of testing (e.g. rectal smears, abdominal radiographs, abdominal ultrasound)have failed to determine a complete diagnosis for an animal's gastrointestinal symptoms.
Many diseases, including: intestinal cancer, inflammatory bowel disease, bacterialinfections, protozoan infections, worms and intestinal viruses, can be diagnosedby intestinal biopsy. In difficult-to-diagnose cases of coccidiosis, bradyzoite and tachyzoite protozoan clusters (the asexually replicating coccidia organisms) can sometimes be seen inside affected intestinal epithelial cells, on tissue biopsy sections. Free-living coccidial organisms (the protozoans that are located outside of the intestinal cells, looking for new cells to invade) can often be seen in biopsied regions of severe intestinal ulceration(places where the epithelial cells have fallen away): organisms are clearly visible amongst the damaged cells and cell debris. In the case of coccidiosis diagnosis, multiple areas of the intestine and colon need to be sampled because the coccidial organisms may onlyinvade a few, localised sections of intestinal wall.
How to take the biopsies:
Biopsies of the stomach, upper small intestine (duodenum) and colon can be taken frominside the intestine or stomach using endoscopy or colonoscopy (cameras inserted along the intestine, via the mouth or anus entry-points) and biopsy forceps (small clamps thatbite off samples of the intestinal or colon lining). Alternatively, biopsies ofany section of intestine (as well as other organs such as the liver, pancreas and kidney) can be taken by opening up the animal's abdomen and sampling the organs from inside ofthe abdomen (this procedure is called an exploratory laparotomy ('ex lap') and biopsy). Exploratory laparotomy is often superior to endoscopic biopsy procedures because, although it is moreinvasive, it allows the vet to clearly visualise the entire abdomen; selectively biopsythe regions of the intestine that look most diseased (and which are, thus, more likely to yield conclusive, diagnostic results); biopsy all layers of the intestinal wall (endoscopy only takes the intestine lining, not all layers of the intestine) and identify specific lesions (e.g. focal intestinal cancers) that might not be seen on endoscopy.
5e) Additional testing - when coccidia can not be found.
Coccidiosis is only one of the diagnoses that might be made in an animal that presentswith symptoms of enteritis, colitis and intestinal disease. Usually, if an animal truly does have coccidiosis as the cause of its diarrhea and colitis symptoms, then fecal flotation, fecal smears and/or intestinal biopsy should be ample to diagnose it.
As will be discussed in the next section (section 6), however, there are many diseases which can produce symptoms of intestinal disease, similar to coccidiosis. Although many of these other non-coccidial diseases can also be diagnosed using the testing procedures already mentioned (fecal float, fecal smears, colonoscopy, exploratory laparotomy and intestinal biopsy), some of these differential diagnoses alternative diagnoses)need special tests to diagnose them. These specific diagnostic tests will be discussed in detail on thepages relevant to the disease processes in question but, for completeness, I have mentionedsome of these alternative tests here.
Fecal smear: a moist cotton tip is rubbed on the animal's rectal wall lining, just inside of the animal's anus. The cotton tip is then rolled onto a microscope slide (making a 'smear' of feces and rectal-lining cellson the slide) and the slide is dried and stained in special stains. Once dry, the slide is examined under a microscope for the presence of diagnostic intestinal cells,inflammatory cells, bacteria and other organisms. Organisms such as Clostridiuminfection, Campylobacter and Giardia infection can be found on rectal smears, along withdiseases such as inflammatory bowel disease (not as accurate as biopsy though) and occasionallyeven intestinal lymphoma.
Images of rectal smears seen under the microscope:
Image 1: This is a fecal smear of Campylobacter organisms as seen under a microscope.The Campylobacter are the wiggly zig-zag lines marked with green arrows: they are often described as being shaped like flying birds (seagulls). These particularorganisms came from a puppy with severe, mucussy diarrhea and bright red blood in the faeces. Notethat, because other non-Campylobacter, spiral-looking organisms can sometimes be found in the faeces ofanimals, Campylobacter should always be confirmed using a fecal bacterial culture (next point).
Image 2: These are Clostridium spores (the white-filled circles marked with arrows) taken from a rectal swab of a dog with diarrhoea. This animal had symptoms of colitisafter eating roast lamb.
Images 3 and 4: Giardia. The outline of the organism, along with its tail is marked in white.Its paired nuclei are marked in blue. Its flagellae (small 'hair-like' structures that it swims with) are marked in green.
Fecal culture: feces can be cultured at the laboratory to detect whether they containpathogenic (disease-causing) bacterial types such as Campylobacter, Salmonellaand Yersinia.
Fecal ELISA: ELISA tests are advanced testing procedures used to detect the presenceof specific organism antigens (proteins) in fecal samples. Fecal ELISA tests have been developed for parvovirus and coronavirus infections, as well as for Giardia and Cryptosporidium:two parasitic organisms that are notoriously difficult to detect using normal fecal assessment techniques.Fecal ELISA tests have also been developed to detect nasty tapeworms like Echinococcus (hydatid tapeworms).
Fecal PCR: PCR is a highly-advanced technique for detecting the presence of specific parasite or infectious organism DNA in tissue and fluid samples. It is highly sensitive and able to detect very small amounts of any organism. PCR tests have been developed for Giardia and Cryptosporidium: two organisms that are notoriously difficult to detect using normal fecal assessment techniques.
Serology - antibody detection: when an animalbecomes infected with an infectious organism or parasite, its immune system produces specificantibodies designed to enhance the killing and clearance of that organism from the animal's body.These antibodies can be detected in the animal's blood and may be used to make a diagnosis of a specificinfectious disease organism. Antibody detection tests have been designed for many infectiousdisease organisms, including: Toxoplasma, Neospora, S. neurona.
6. What other diseases look like coccidiosis in dogs and cats?
Coccidiosis is characterized by irritation and inflammation of the small intestine and sometimes the colon, resulting in symptoms of enteritis and colitis: watery to semi-solid diarrhea; slimy, jelly-like stools; stinky,putrid-smelling stools; fresh blood in the faeces; increased frequency of defecation; straining to defecate;dehydration and occasionally weight-loss, wasting, weakness, pale gums and vomiting.
Sounds simple enough ...
Unfortunately for those of you who like to self-diagnose, there is a wide range of other conditions that dogs and cats can get which may also present as symptoms of intestinal disease. Basically, any condition that causes severe irritation or inflammation of the small intestine and colon will produce these clinical signs. The followingis a list (by all means not exhaustive) of conditions that can present similarly:
Inflammatory Bowel Disease (IBD) - a common, allergy-initiated, auto-immune condition whereby inflammatorywhite blood cells (lymphocytes, plasma cells and/or eosinophils) attack the walls ofthe intestines and colon resulting in symptoms of gastroenteritis and colitis. Proteinsfrom the animal's food or proteinaceous bacterial antigens are thought to be the underlying cause.
Whipworm - Whipworms (Trichuris vulpis) live in the colon, sometimes producingsigns of colitis.
Intestinal worms - Round worms, hook worms and tape worms live in the small intestinesof dogs and cats, sometimes producing symptoms of gastroenteritis in young animals (weight loss, diarrhoea, wasting).
Bacterial overgrowth - certain species of bacteria (especially Clostridium and E. coli species)live in the intestines all of the time without causing any disease issues. They normally only become a problem when their numbers are permitted to grow out of control. This generally occurs when the animal is allowed to gorge itself on rich foods (e.g. lamb and pork are huge culprits) or ingest fouled garbage (stuff from thebin, semi-rotten, spoiled foodstuffs, animal carcasses).
Bacterial pathogens Some bacterial organisms preferentially invade the intestines and colon,causing signs of gastroenteritis. These include: Salmonella, Yersinia, Campylobacter and Clostridium. These organisms infect dogs and cats through the consumption of another animal's faeces or through the consumption of old/aged, undercooked or contaminated foodstuffs (e.g. food poisoning).
Giardia and Cryptosporidium. - semi-common, fecally-ingested protozoan organisms that can producea range of intestinal symptoms. The organisms usually come from contaminated waterways.
Viral enteric diseases. nasty intestinal viruses such as canine parvovirus, feline panleukopenia, canine distemper, canine coronavirus and rotavirus can produce symptomssevere gastroenteritis: vomiting, diarrhoea, weightloss, abdominal pain, dehydration, bloody stools etc.
Diet changes - abrupt diet alterations, particularly in young animals, can commonlyproduce symptoms of gastrointestinal upset - vomiting, diarrhoea, inappetence etc.
Intussusceptions - the intestine of some animals can telescope insideof itself, producing symptoms of straining to defecate, blood in the stools, vomiting, inappetence,weightloss and slimy droppings. Diagnosis is made by ultrasound, colonoscopy or exploratory surgery.
Constipation - constipated animals can present with signs of straining, bloody discharges from the rectum and anus and slimy, jelly-like anal discharges.
Intestinal masses - animals with intestinal, colon, rectum and anal cancers can present with signs of diarrhea, weight loss, straining to defecate, bloody discharges from the rectum and slimy, jelly-like anal discharges.
Intestinal foreign bodies - animals with intestinal, colon, rectum and anal foreign bodies can present with signs of vomiting, inappetence, weight loss, straining,bloody discharges from the rectum and slimy, jelly-like anal discharges. Common foreign bodies include cooked bones, corn cobs, stone-fruit pits and pieces ofingested stick.
7. Coccidiosis Treatment - how is coccidia in puppies and kittens and adult animals treated?
Most cases of coccidiosis in dogs and cats that present to a veterinary clinic are relatively non-complicatedcases. The puppies and kittens are bright and alert and interactive and normally stilleating, but they are producing vile-smelling, watery, slimy-looking stools; defecating more often than normal and occasionally producing flecks of blood in their droppings. Most of these non-complicated cases will be treated at home, using anti-coccidial remedies (section 7a), antibiotic-type medications (see section 7b) and dietary management (7e).
Occasionally puppies and kittens will present to the vet in a more critical state, with inappetence, weakness, severe dehydration and even anaemia and shock. These animals willneed to be cared for more intensively and often need to be admitted into hospital forintravenous fluid support (7c) and even blood transfusions, in addition to the other treatmentmodalities mentioned above.
As a general rule, the treatment of canine and feline coccidiosis is pretty much a treatment of the organism itself and management of its associated symptoms. The aim of therapy is to keep the animal comfortable, provide treatments that will kill offmost of the coccidia organisms and manage and prevent any secondary complications that might occur(e.g. secondary bacterial infections, dehydration, anaemia, rectal prolapse). The hope is that, eventually, the animal's own immune responses will take over and control the coccidia's numbersand replication, thereby bringing the condition to a conclusion. The treatment options for coccidiosis, their mechanisms of action and their pros and cons are discussed below.
7a) Antimicrobials - coccidiostats (drugs that inhibit coccidial growth).
Anticoccidial medicines are pretty much the mainstay of coccidia treatment. Thesemedications are designed to reduce the numbers of coccidial organisms present within theanimal's intestines and, therefore, reduce the damage that they are able to cause whilst theanimal's immune system is attempting to take back control of the situation. Although somecoccidial medications may occasionally clear coccidia from the gut completely, most coccidialmedications really only buy the animal time. Few of them completely eradicate the coccidial organismsfrom the intestines - they just keep the bugs at bay whilst the immune system isre-establishing the balanced state (premunition) between the host animal and the organism (the non-clinical carrier state). It is thought that these drugs may wellspeed up the onset of premunition and shorten the symptomatic period of disease.
A lot of vets only prescribe anti-coccidial remedies to their 'well' coccidia patients and nothing else. Puppies and kittens that present to the veterinarian with diarrhoeaand abnormal faeces, but which are otherwise bouncy and bright and eating - these animals often do perfectly fine on anti-coccidial medications alone.
Animals that present to the vet with lots of blood in their stools, reduced appetitesand severe dehydration often require treatments in addition to anticoccidial medications(e.g. antibiotics, fluids).
An important note on treatment: when dealing with litters of young puppies or kittens, do not only limit treatment to the sick creatures. Treat the whole litter and any otherunderage animals that have come into contact with them. You can expect transmission tohave occurred.
Older animals that test positive (on a fecal float) for coccidia, but which have noclinical signs of the disease do not need to be treated. These animals are probably immuneto the disease, but are non-clinical carriers. Treatment won't stop their carrier stateso treatment is not really worth it.
Commonly used coccidiostats (anti-coccidial drugs) include:
1) Trimethoprim sulfonamide combinations (trade names include Tribrissen, Trivetrin):
Trimethoprim and sulfonamide are two individual antibiotic drugs that have been found to havesynergistic properties (enhanced organism killing properties) when they are used together. These drugs are the first choice (drugs of choice) for coccidiosis treatmentand are thought to be less toxic than many of the other antimicrobial chemicals used for this purpose.
In addition to suppressing coccidial growth and replication, trimethoprim sulfadrugs are also able to kill many of the intestinal bacterial types that come insecondarily and complicate coccidial infections. These include: Streptococcus, Staphylococcus, E. coli, Klebsiella, Clostridium, Proteus and Salmonella. They have poor action against Pseudomonas. The trimethoprim-sulfa drugs are particularly useful because they are able to access many tissues (including the brain) that many other antimicrobial combinations can not reach.
Some cautions: trimethoprim/sulfonamide (TRMS) drugs should be avoided or used very cautiously in very young animals, very old animals, animals with kidney issues and in very dehydrated animals because they have the potential to cause renal problems (crystals of TRMS will deposit in the kidneys, leading to renal injury).TRMS has the potential to cause dry-eye (keratoconjunctivitis sicca - KCS) in susceptible individualsand caution should be exercised, particularly in KCS-prone breeds like cocker spanielsand cavalier king Charles spaniels. Trimethoprim/sulfonamide combinations should be avoidedin certain breeds, especially the dobermann and weimeraner, because of the risk of druginduced autoimmune joint disease and skin disease.
Dose rate: 15-30mg/kg orally, once to twice a day if the pet is a cat or a dog under 4kg. Two references [23,25] suggested a dose rate of 15mg/kg twice daily for cats.
Dose rate: 30-60mg/kg orally, once a day if the pet (dog) is over 4kg. Note that the 4kg specification was only mentioned in a single text [ref 2] and that most other textbooks referenced here mention only the 15-30mg/kg dose rate (regardless of weight).
The trimethoprim-sulfonamide combination is generally administered for 5 days [ref 2, 22, 23].
According to Nelson and Couto [ref 1], the drug may be given for 10-20 days.
Another reference suggested giving the drug for 5-20 days, the length of time beingdependent on the animal's condition. The suggestion was to treat until the animal'sclinical signs had abated and/or until no oocysts could be detected in the faeces on fecal flotation. This would seem to make most sense from a veterinary perspective.
Furazolidone is a nitrofuran drug (nitrofurans are another antibiotic drug family) that can be used to treat coccidiosis, either on its own or in combination with the trimethoprim sulfa drugs.
Dose rate: 8-20mg/kg orally, once to twice a day (cat or dog) [ref 2,23,29,31] .
It is generally administered for 5-7 days.
Halve the dose when using furazolidone in combination with the trimethoprim sulfa drugs.
3) Amprolium (trade names include Coccivet):
Amprolium is another anticoccidial remedy that can be used to treat coccidiosis in dogs and cats. As seen in the product image (right), it is also one of the more common medications usedto treat coccidiosis in birds.
Dose rate: no greater than 300-400mg total dose orally per day (given once daily) for dogs [ref 2,23]. The drug is usually given for 7 days (can extend up to about 12 days) to dogs. The Veterinary Drug Handbook [Ref 30] suggests 100mg total dose once daily for 7-12 days in puppies.
Dose rate: no greater than 60-100mg total dose orally per day (given once daily) for cats [ref 2,23,30] .
Amprolium is generally administered for 5-7 days in a row [ref 1,2,23,30]. According to one reference (Nelson and Couto - 1), Amprolium can be associated withtoxicity in cats. Because of this, I would consider it a last choice option in this species.
Important note: Although this product (Amprolium) has been used successfullyin many dogs and cats with coccidiosis and is referenced in many top-quality veterinary textbooks, we must make you aware that this product is not currently registered for use in dogs and cats (i.e. it is being used off-label and in a way different to that for which it was designed). Consequently, your veterinarian, the drug company manufacturer and this website (us) can notbe held accountable should you decide to use this product in your feline or caninecompanion and should you experience any adverse side effects with it.
Author's note: I have no personal experience with Amprolium in dogs and cats, only inbirds and poultry. I would personally avoid it in cats, due to the unknown toxicity features.
4) Toltrazuril (trade names include Baycox):
Toltrazuril is another anticoccidial drug that is commonly be used to treat coccidiosis in dogs and cats. As seen in the product image (right), it is also one of the more common medicines usedto treat coccidiosis in piglets.
The piglet preparation pictured on the right is the Toltrazuril productthat our clinic uses in coccidiosis-affected dogs and cats in our own clinic. Most of the clinics that I have worked in within Australia have stocked this product for the treatment of coccidiosis in dogs and cats - its use is widespread.
Dose rate: 15mg/kg (range 5-20mg/kg) orally, once a day (cat or dog)[ref 2,29]. A dose rate of 30mg/kg once daily for 3 days has been suggested for cats [ref 29].
Dose rate: the piglet suspension pictured is commonly given at 0.4ml/kg orally, once a day (cat or dog).
It is generally administered for 3-5 days in a row [ref 2].
It is available as a syrupy suspension for ease of administration.
There is also an injectable form of Toltrazuril available for animals who are not taking oral medications.
Important note: Although this product (Toltrazuril and Baycox Piglet) has been used successfullyin many dogs and cats with coccidiosis, we must make you aware that this product may not be currently registered for use in dogs and cats (i.e. it may be being used off-label and in a way different to that for which it was designed). Consequently, your veterinarian, drug company manufacturer and this website (us) can notbe held accountable should you decide to use this product in your feline or caninecompanion and should you experience any adverse side effects with it.
Author's update 21/11/09: A recent conference attended by the author discussed the treatment of coccidiosis in dogs and cats in animal shelter situations. Toltrazuril (Baycox) was considered by most shelter vets in attendance to be a highly effective treatment for the control of coccidiosis. Not only does it seem to work, but it also comes as a liquid formula for easy dosing, it is palatable and it only needs to be given once a day for about 3-5 days.
5) Other drugs:
Other drugs, including Quinacrine, Spiramycin, Ponazuril and Roxithromycin have been trialledin dogs and cats with coccidiosis with variable success. Ponazuril, a close relativeof Toltrazuril, may be of use in cats and horses afflicted with the neurological coccidial disease Sarcocystis neurona.
Ponazuril - 7.5-15mg/kg once daily for 1-3 days in dogs and cats. [ref 2,29]
Diclazuril - 25mg/kg once daily for a single dose in the cat. [ref 29]
Quinacrine - 10mg/kg once daily for 5 days in the cat. [ref 2,32] This drug is not longer available in many countries, including the USA.
Important note: It has been brought to the author's attention that some of our readers have been using this page to self-diagnose and self-treat coccidia problems in dogs and catswithout first seeking veterinary attention. PLEASE DO NOT DO THIS. Although this website makes every attempt to be accurate and up-to-date - recommended drug treatments, drug doses, drug durations and drug-safety recommendations change constantly and we may not always be able to keep up with the most-current knowledge. Some of the drugs listed on this page have important side effects and not all drugs and doses will be safe for all individuals (e.g. some dose rates may be toxic in some individuals). You must get your vet to diagnose coccidia in your pets (do not self diagnose) and recommend the best and safest treatments for your situation.
Please read our disclaimer. This website (us) is designed for information purposes only - so that you will know what options might be available to you and what questions and topics to ask your own vet about. It is not designed to replace your own vet's opinion. Your vet can directly examine your animal - we can not. This website will not be held accountable should you decide to use any listed product in your pet and should you experience any adverse side effects with it.
We have removed the dose rates for albendazole and fenbendazole out of a concern for our readers' pets. Despite our warning about the off-label nature of these two drugs; our disclaimer and our warnings about the risks of self diagnosis and the self-dosing of pets, people have been dosing their pets with these two drugs without direct veterinary advice and guidance. Neither drug is particularly good at treating coccidiosis compared to most of the other drugs mentioned, so their inclusion in the list is non-essential. The risks of their use far outweigh the benefits - hence we have removed them. Please be advised that all of these listed drugs (e.g. ponazuril, trimethoprim-sulfa, amprolium and so on) can have nasty side effects in certain individuals. Albendazole, in particular, can have very serious, even life-threatening side effects and should never be dosed without veterinary guidance.
Once again, please do not treat your own pets for any diseases without direct veterinary guidance. Only vets who have directly examined your pet can tell you which drugs and doses are most effective and safe for your animal. No website can guarantee this like your own clinician can.
Although some of these coccidial treatments are available as formulations for mixing into animal feedsand water-sources, it is always best that you dose affected animals individually,if possible. Sick animals are unlikely to drink or eat as well as healthy animals and might, therefore, miss out on their share of much needed antimicrobial drugs.
Some livestock feeds and preparations contain other coccidiostatic compounds such asMonensin and Lasalocid and Decoquinate. These compounds should not be given tocats or dogs. Monensin and Lasalocid are very toxic to horses and should never be fedto this animal species.
7b) Antibiotics - drugs used to kill secondary bacteria.
The use of antibiotics in animals that are affected with clinical coccidiosis is variableand varies from vet to vet and case to case. Some vets use them in addition to the coccidiostatic drugs and some vets do not.
As mentioned in section 4b, animals with coccidiosis suffer a lot of damage and inflammation to thelining of their gastrointestinal tracts and are, therefore, at risk of developing severe secondary infections from bacteria present in the intestine and colon. If it is severe enough, this infection can potentially lead to bacterial infection of the blood and other organs, resulting in septic shock and even death for the patient. Antibiotic medications may be a valid form of additional treatment for these coccidia patients because they may prevent secondary bacterial infections from occurring.
Personally, I would always give antibiotics to coccidia patients that are very unwell;that are febrile (feverish); that have a very high or low white blood cell count; that have toxic changesin their white blood cells or that have a lot of blood in their faeces. Put it this way, if bloodcan get from the blood vessels into the intestines (seen as blood in the poo), then bacteriacan surely get from the intestines into the blood. Patients that are very bright and alertand well-looking might not need additional antibiotic cover, however, I would give them antibiotics if there was a lot of blood in the stools.
Commonly used antibiotics include:
1) Trimethoprim sulfonamide combinations (trade names include Tribrissen, Trivetrin):
Again, the trimethoprim and sulfonamide (TRMS) combination drugs are an excellent choice inthese animal patients (provided these patients are not too dehydrated, debilitated, young or old). These drugs have the best effect against coccidia and they are highly effective at killing many of the secondary bacterial types that commonly complicate gastrointestinal infections, including: Streptococcus, Staphylococcus, E. coli, Klebsiella, Clostridium, Proteus and Salmonella. Using TRMS drugs in animals with coccidiosis is handy because it kills two birdswith one stone: TRMS takes care of both the primary coccidial organisms and the secondaryinvaders, thereby avoiding the need for owners to give two separate types of antibiotics totheir canine and feline pets.
Additionally, the trimethoprim-sulfa drugs are particularly useful because they are able to access many tissues (including the brain). This makes them a potential future choicefor emerging protozoan brain infections such as Sarcocystis neurona.
Remember these cautions: trimethoprim/sulfonamide (TRMS) drugs should be avoided or used very cautiously in very young animals, very old animals, animals with kidney issues and in very dehydrated animals because they have the potential to cause renal problems (crystals of TRMS will deposit in the kidneys, leading to renal injury).TRMS has the potential to cause dry-eye (keratoconjunctivitis sicca - KCS) in susceptible individualsand caution should be exercised, particularly in KCS-prone breeds like cocker spanielsand cavalier king Charles spaniels. Trimethoprim/sulfonamide combinations should be avoidedin certain breeds, especially the dobermann and weimeraner, because of the risk of druginduced autoimmune joint disease and skin disease.
Dose rate: 15mg/kg orally, twice a day. It is generally administered for 10-14 dayswhen used in an antibacterial capacity (5-20 days for coccidia). Note that larger dose ratesare used when killing coccidia.
2) Clavulanic acid/amoxycillin combinations (tradenames include Augmentin, Clavulox, Noroclav):
Many of the bacterial infections that complicate simple cases of enteritis, colitis and coccidiosis arequite responsive to the antibiotic, amoxycillin. Amoxycillin is a B-lactam antibiotic, closelyrelated to penicillin. Because some of the strains of Streptococcus and Staphylococcusare now resistant to amoxycillin on its own (these bugs make penicillinase, an enzyme which destroys the drug), this antibiotic is generally combined with a secondary antibiotic called clavulanic acid, which helps to overcome this bacterial resistance (it inhibits penicillinase).
Amoxycillin is effective against a range of bacterial pathogens that commonly complicate enteritis and colitis, including: Streptococcus, Staphylococcus, Corynebacterium, E. coli, Klebsiella, Salmonella, Campylobacter, Clostridium and Proteus. Amoxycillin/clavulanic acid combinations are safe to use in most dogs and cats, including young puppies and kittens, pregnant and lactating bitches and queens and dehydrated or very sick animals.
Dose rate: 15-20mg/kg orally, twice a day. It is generally administered for 10-14 days.
Clavulox is also available as a syrup for easy administration.
Image - Clavulox can be used in animals with small and large intestinal diarrhea.
3) Enrofloxacin, orbifloxacin and other fluoroquinolones (trade names include Baytril and Orbax):
The fluoroquinolones are one of the newer antibiotic families to appear on the veterinary market.These are great drugs with a good spectrum of action against many of the bacteriathat complicate coccidiosis, including: E. coli, Klebsiella, Clostridium, Proteus, Campylobacter, Yersinia and Salmonella. They even kill Pseudomonas. They tendto be more expensive than many of the aforementioned antibiotics (especially in large dogs)and, because of their relative 'newness', many vets choose to use them as a last resort (reserved for severe infections only) in order to limit the evolution of bacteriathat are resistant to them. Some bacteria are only susceptible to fluoroquinolones nowand we are trying to reduce the development of antibiotic resistance to these drugs by not overusing them.
Cautions: Fluoroquinolones are safe for most animals, however there are some side effects thatneed to mentioned. These drugs should not be used in dogs under 12months of age (under 18 months of age for large breeds) because of toxic effects that the drugs have on growingbone cartilages. They should be avoided in cats of all ages, where possible, because of links withsudden retinal atrophy and permanent blindness. Seizures and strange neurological signshave been occasionally seen in animals subjected to high-level, prolonged dosing with fluoroquinolones. It is possible for fluoroquinolones to crystallize in the urine and to cause renal failure(particularly if used at very high doses) and, therefore, these drugs should be used cautiously in very old animals, very debilitated animals and animals with pre-existing renal compromise.Fluoroquinolones can potentially be associated with tummy upsets (inappetence, vomiting and diarrhea) and so owners need to watch out forthese signs and call their vet if they occur.
Dose rate: Baytril - 5mg/kg orally, once a day. It is generally administered for 10-14 days.
Baytril is available as a syrup for easy dosage.
Baytril can be given subcutaneously and even intravenously in sick or inappetent or vomiting animals.
Images of the fluoroquinolone drugs, Baytril and Orbax.
4) Metronidazole (trade names include Metrin, Metrogyl, Flagyl and Metronidazole):
Metronidazole is an antibiotic drug that is commonly given to animals with diarrheabecause it treats against a broad range of intestinal and colonic bacteria and protozoa, including Giardia, Clostridium, Campylobacter and other enterobacteria. It isnot particularly useful for treating coccidia-type protozoans, however, which is why it is includedin the antibacterial section and not in the anticoccidial section.
Metronidazole is also thought to have an anti-inflammatory effect on intestinal tissues and may go some way towards reducing the symptoms of inflammation and discomfortseen in patients with severe enteritis and colitis.
Cautions: Metronidazole is safe for most animals, however there are some side effects thatneed to mentioned. Seizures, vestibular disease symptoms and other strange neurological signs have been seen in animals subjected to high-dose-rate, prolonged dosing with metronidazole. These signs may be reversible if the drug is discontinued.Metronidazole can potentially be associated with tummy upsets (inappetence, vomiting and diarrhea can result) and so owners need to watch out forthese signs and call their vet if they occur. The oral preparations of metronidazole taste particularly foul and can put some animals (particularly horses) off their food if given orally.
Dose rate: 15-25mg/kg, orally, twice daily. It is generally administered for 10-14 days.
Metronidazole is available as a syrup (Flagyl suspension) for easy dosage.
Metronidazole can also be administered intravenously or subcutaneously in sick or inappetent or vomiting animals.
Image 1: Metrogyl is one form of Metronidazole tablet available on the market for our furry friends.
Image 2: The intravenous Metronidazole BP preparation.
5) Doxycycline and other tetracycline drugs (trade names include Vibravet):
The tetracycline drugs, particularly doxycycline, are used in the treatment of a wide range of canine andfeline infectious disease conditions. They are effective against a very broad varietyof infectious disease organisms and their spectrum of action includes many of the organisms commonly implicated in gastrointestinal diseases. As an extra bonus, the tetracycline antibiotics are able to penetrate a wide range of tissue types and gain access to the inside of animal cells (which aids in the killing of hard-to-reach intracellular bacteria).They are also thought to have an anti-inflammatory effect on intestinal tissues and thismay go some way towards reducing the symptoms of inflammation and discomfort experiencedby patients with enteritis and colitis.
Cautions: Tetracycline drugs are quite safe for most dogs and cats of any age, however there are some side effects that need to mentioned. Tetracycline drugs have the potential to cause browning of the developing permanent teeth of an animal, when given to animals under 4 months of age. For most owners, this staining will not be of concern, but owners of show animals might want to be mindful of the potential. Doxycycline can potentially be associated with stomach ulceration and tummy upsets (inappetence, vomiting and diarrhea can result) and so owners need to watch out forthese signs and contact their vet if they occur. It is sometimes possible for tetracyclines other than doxycycline to cause renal failure and, therefore, these drugs should be avoided in very old animals, very debilitated animals and animals with pre-existing renal compromise.
Dose rate doxycycline: 5mg/kg orally, twice a day. It is generally administered for 10-14 days.
Vibravet is available as a paste for easy administration.
Important: Doxycycline tablets should be washed down with water (via a syringe) after giving sothat they go all the way to the stomach and do not sit in the esophagus. This is importantbecause doxycycline tablets and capsules have been known to cause severe oesophageal ulcersand strictures if they get stuck here.
Oral tetracycline drugs should not be given with food that contains calcium (e.g. milk, bones)or magnesium or other cations because these reduce its absorption from the gut.
Images of VibraVet, a doxycycline formulation.
6) Other drugs:
Tylosin at 10-25mg/kg three times daily for 2-3 weeks has been found to be effectivein treating a range of colonic and enteric infections.
Chloramphenicol has also been used in treating colonic bacterial infections - it has severe human health implications (aplastic anaemia) and should be only used as very last resort.
7c) Intravenous Fluid Support.
1) Intravenous Fluids:
Kittens and puppies that develop severe coccidia-associated enteritis and colitis are at risk of temporarily losing one of the intestine's major functions: the absorption of water from theintestinal tract. Not only are these animals unable to absorb the water thatthey drink, they are also pouring massive amounts of inflammatory fluid, intestinal-secretory fluid and moisture-containing mucus out of the walls of their injured bowels, into their faeces. Severe, watery diarrhea results and these young animals will often dehydrate very quickly. Ownerswill think their pup or kitten has suddenly lost weight, it has shrivelled away soquickly from dehydration.
Often these animals are losing so much water from their intestines that they are completelyincapable of drinking enough water to keep up with the losses. Additionally, much of whatthey are consuming, water-wise, is not being absorbed into the body anyway because their intestinal water absorption is so impaired. Much of the water ingested goes straight out the back end. Puppies and kittens with severe coccidiosis (especially patients with severe colitis) almost always need intravenous fluid support. It is the only way to get fluids into these animals when they are unable to properly absorb water from their intestines.
Author's note: sometimes, puppies and kittens come into the vet that are so collapsed and severely dehydrated (with severe shock signs), the veterinarian can't get an intravenous catheter into their leg veins. The blood vessels in their legs have shrivelled down to such tiny, thin tubes that no intravenous catheter will thread up them. In these cases,the vet will sometimes be forced to put a catheter into the pet's jugular (the large vein in the neck) or insert a needle into the bone marrow of the animal's thigh (femur) or upper arm (humerus) to administer the fluids. Don't be alarmed to see your vet do this: it looks brutal, but these techniques are perfectly acceptable ways of giving lifesaving fluids to young animals in severe shock.
As mentioned in section 4b, often severe diarrhea is associated with a marked loss of blood salts (called electrolytes) into the intestines. These losses can be replaced by placingelectrolyte supplements into the intravenous fluids that are given to the young animal. Glucose levels can also become very low in puppies and kittens with severe gastroenteritis andsecondary bacterial infections (the body's glucose gets gobbled up by multiplying bacteria and by the inflammatory cells that come in to treat the disease) and so glucose may also need to be added to the intravenous fluids to keep the pet's blood glucose levels normal.
Pictures of intravenous fluids and the electrolyte supplements (potassium, phosphate etc.) used to correct electrolyte abnormalities in pets with enteritis and diarrhea.
2) Special intravenous fluids - colloids:
Puppies and kittens with severe ulceration and inflammation of the intestines can lose massiveamounts of inflammatory fluids and blood proteins out into their faeces (inflammatory fluidscontain large quantities of blood-derived proteins). Over a matter of days, these losses can accumulate, resulting in a massive drop in the animal's overall blood protein levels, a deficit that is further exacerbated should the animal go on to develop severe intestinal bleeding (blood contains lots of protein - intestinal and colonic bleeding can result in loads of blood protein pouring out of the pet's bowel, into its faeces).
When dogs and cats lose excessive amounts of protein into their intestines, their overallblood protein levels can plummet. Of particular importance is a protein called albumin. Severe drops in blood albumin levels can have severe repercussions for the animal's health, body-functions and survival. Albumin has a large number of essential functions within the body,one of the most important being the maintenance of colloid-oncotic pressure (COP). Colloid-oncotic pressure (COP) is a force that is exerted by the charged particles (e.g. blood salts) and heavy molecules (e.g. albumin) that drift in the bloodstream, which acts to keepthe watery fluid present in the blood (the plasma) within the blood vessels. Without this COP force, the blood's fluid will leak out of the blood vessels, resulting in a severe drop in the animal'sblood pressure (i.e. not enough blood for the organs) and infiltration of watery fluid into areas of the body where it is not wanted (e.g. the lung and the brain!)
When albumin is lost during severe coccidial infections, there is a risk of the animal'scolloid-oncotic pressure falling. Veterinarians treat this situation by either:
1) giving the patient special, synthetic, intravenous fluids, which contain heavy molecules that mimic the COPeffects of albumin (these special fluids are termed colloids) or
2) replacing the lost albumin with real albumin by giving the animal a plasma transfusion (see next point).
Colloids can also be given to puppies and kittens that come in to the vet clinic with severe signs of shock and low blood pressure, regardless of their blood protein levels. Because they act to increase the colloid oncotic pressure inside the animal's blood vessels, they can act to draw fluid into the blood vessels from other areas of the body, thereby boosting the animal's blood pressure rapidly whilst the animal is being properly and concurrently hydrated with other fluids.
Cautions: Colloids are great products, but they don't come without risks. They are artificial synthetic substances and, assuch, they have the potential to cause allergic and anaphylactic reactions in patientsgiven them. Colloids also have the potential to instigate bleeding problems in animals that have low blood platelet levels and they can sometimes cause an animal's blood pressure to become too high(adding to bleeding risks).
3) Special intravenous fluids - plasma:
Plasma is the fluid that remains when you remove all of the cells from blood. It containsseveral helpful substances that can really make a huge difference to the survival chances of coccidia puppies and kittens.
1) albumin - as mentioned before, albumin is responsible for maintaining colloid oncotic pressure within the bloodstream of puppies and kittens so that their blood vesselsdon't start leaking. Albumin is also responsible for many other functions withinthe body, including the transport of certain hormones, antibiotics and therapeutic drugs throughthe bloodstream.
2) immune-modulatory proteins (e.g. some inflammatory proteins) - these assist the bodyin generating an effective immune response.
3) proteins that mediate blood coagulation - many severely septic animals (e.g. animals with secondary bacterial invasion of the blood) developlife-threatening disorders of their normal blood clotting mechanisms: a problem termed DIC (disseminated intravascular coagulopathy). In DIC, the patient's bloodclottingmechanisms are disrupted: the blood either wants to clot excessively, leading to blood-clots, embolisms and 'strokes' or, alternatively, it does not want to clot at all, leading to excessive bleeding. Plasma contains the necessary blood-clotting regulatory compounds required to treat this critical complication.
Plasma is more expensive than the synthetic colloids are, however, it contains many helpful elements in it that the synthetic colloids do not. Plasma is the only source of'true' albumin replacement for an animal that vets can give. Although the synthetic colloidsdo do a good job of replacing COP, they can not replace the many other functions thatonly 'true albumin' can do (e.g. drug transport).
Author's note: a lot of plasma (many bags of it) needs to be given to a large dogto generate any noticeable shift in its overall protein and albumin levels. This can getexpensive. Fortunately, most of our severe coccidia patients are young and small (under 4kg)and, therefore, smaller quantities of plasma are able to make a bigger, more noticeabledifference, in their protein levels, thus reducing the costs of treatment.
Cautions: Also note that plasma, like the synthetic colloids, is not without risk. It is aproteinaceous substance being given intravenously. Consequently, there is the possibility that a plasma transfusion could cause allergic or anaphylactic reactions in some patients,particularly if the patient in question has received plasma, blood or tick or snakebite antiserumpreviously.
7d) Special intravenous fluids - blood:
Some patients lose so much blood as a result of gastrointestinal and colonic ulceration that theycan actually become severely anemic in addition to losing protein into the gut.
These patients may require a blood transfusion (containing red blood cells)to replace those red blood cells lost into the gut and faeces. If whole, fresh bloodis available (blood taken freshly from a donor within the last few hours), this can be particularly helpful to the patient. In addition to red blood cells, freshly-drawn, whole blood contains much-needed albumin, plasma antibodies and also live white blood cells. Septicaemic dogs and cats often have low white blood cell counts (the cells are lost fightingthe overwhelming infection) and, consequently, live white blood cells in a bloodtransfusion may be of some help in fighting the infection.
Caution: Please note that blood transfusions, like synthetic colloids and plasma, are not without risk. Blood is a proteinaceous substance being given intravenously. Consequently, there is the possibility that a blood transfusion could cause severe (even fatal) allergic or anaphylactic reactions in some patients (particularly cats), particularly if that animalin question has received plasma, blood or tick or snakebite antiserum previously.
Generally, most dogs can receive a first blood transfusion from any canine donor withoutthe need for cross-matching of blood. Subsequent transfusions can also be givenwithout cross-matching, if they are given within 48 hours of the first one. Cross-matching is required for any blood transfusion given 48 hours or more after the first transfusion.
Cats must be blood typed before any blood transfusion, even the first one. Cats have very specificblood types (A and B and AB) and must only receive blood of the correct type. In an emergency (i.e. if a cat donor can not be found), a one-off transfusion of canineblood can be given to a cat.
7e) Diet Management.
Animals that present to the veterinarian with symptoms of coccidial enteritis can often benefitfrom a diet that is bland (won't disturb the gut) and easy to digest. Diets based onbland, skinless, non-fatty, white meats such as rabbit, chicken or turkey (not fish) and bland carbohydratessuch as rice and potato (rice is better) are ideal for the damaged gut.
There are many excellent, fully balanced, veterinary prescription diets available these days, which be given to animals of all ages that are convalescing from any form of intestinal upset. These include, Hills Science Diet i/d (cat and dog forms) and Royal Canin Digestive Diet. Alternatively, you can feed your recuperating pet on boiled, skin-free chickenbreast and well-rinsed rice (rinsing the rice thoroughly after cooking to remove the starch)for a week, while its intestines and colon settle down and heal. This home-made diet is notbalanced and not appropriate for long-term feeding, but it is fine to give for a week or so.Some animals will refuse to eat the bland prescription diets but will happily tuck into home-cooked chicken and rice - it just gives you another option.
Whatever food you choose to give, should be given little and often. Animalswith sick intestines should only be fed small quantities of food(e.g. 1-2 tablespoons) at a time and these amounts should be given frequently - every couple of hours. Large quantities of food entering the injured intestine are more likely to be vomited up or go through the damaged gut undigested and wasted. Smaller quantities are toleratedmuch better. Give the food every few hours so that the pet is getting its daily nutritionalrequirements.
Food can be made more palatable by heating it in the microwave. Don't make it toohot!
Avoid fatty foods when the intestine is injured!
Animals that are vomiting up food will often benefit if they are fasted for 24-48 hours. This rests the intestinal tract and allows it to heal a bit before more food is given. Water should not be withheld.
Animals that are in hospital with severe gastrointestinal signs, anorexia and diarrhea can begiven assisted feeding, either by nasogastric tube (a tube placed from the nose into the stomach)or esophageal-gastric tube (a tube placed from the oesophagus into the stomach). A PEGtube (tube going directly into the stomach) is seldom required.
Intravenous nutrition can be given to animals that are unable to keep down any oral foods or absorb any foods from their intestines. This would seldom be required for most cases of coccidiosis.
8) What is the prognosis for coccidiosis in dogs and cats?
Most cases of clinical intestinal coccidiosis that present to the vet and are treated with appropriateantimicrobial therapies do very well. As a general rule, the prognosis for clinical recovery from non-complicated cases of coccidia infection is excellent. It must be mentioned, however, that, because many of these successfully-treated animals do go on to develop a persistent, non-clinical,carrier state (i.e. they retain live coccidia organisms in their colons), the prognosisfor complete coccidial clearance is moderate to poor. Put simply, the prognosis forrecovery and resolution of clinical signs is excellent, but the prognosisfor clearing the organisms completely from the body is poor.
Very young, malnourished and undergrown animals that present with severe signs of coccidiosismust be considered to have a more guarded prognosis. These animals often have very littlenutritional and immune system reserves to fall back on when they become ill and they are morelikely to succumb to secondary complications such as lack of blood sugar, secondary bacterialinfections and low blood proteins. Surgical complications such as intussusception andrectal prolapse seem to be more common in very young animals too.
Young animals with concurrent intestinal diseases such as intestinal worms, Clostridiuminfection and Campylobacter infection should also be considered to have a poorerprognosis.
Older animals that develop recurrent or long-term (chronic) coccidial infestationsshould also be considered to have a guarded prognosis too. It is not common for clinical signs of coccidiosis to persist in older animals and such animals should be suspectedof having some form of underlying intestinal disease or immune disorder. Failure totreat the underlying causes will result in a failure to clear the clinical signs ofcoccidiosis for any long-term period.
The prognosis for Sarcocystis neurona in cats (and horses) is very guarded.
9) How to prevent coccidia in puppies and kittens and adult pets.
Currently, there are no vaccines available for the prevention of coccidia in puppiesand kittens. As a result, prevention of coccidiosis involves reducing your pet's chances of coming intocontact with contaminated environments and faeces and enhancing the health, body condition and immunity of your animal.
9a) Avoid contact with infected dogs and cats and contaminated environments:
Because most cases of coccidiosis occur when puppies and kittens are exposed to stressful environmentsthat contain lots of dogs or cats all co-existing in close proximity to each other, it stands to reason that coccidiosis can often be prevented by not placing your pet in these situations. Boarding your dog or cat with friends or getting a house-sitter to look after your house and pet while you go on holidays can be a low-disease-risk alternative to boarding your dog or cat in a boarding kennel or cattery. Making sure that your fences and yard are dog-proof and keeping your cat indoors (or in a specialcat aviary) can reduce the risk of your friend getting out and ending up in a pound or shelter wherecoccidiosis is rife.
Sometimes, of course, you do have to place your pet in environments where there aremany other dogs and cats and thus the potential for parasitic disease spread. There are precautions that you can take, however, to reduce this risk. Some handy tips include:
1) Vaccinate your pet within 6 months of attending a show event or boarding kennel/cattery:
Although vaccines do not protect your pet against coccidia, it is important thatyour pets are protected against vaccinatable diseases when they go to kennels andcatteries. Animals need a strong immune system to fight off and prevent coccidiosisand making sure that your pet does not contract some other preventable disease(e.g. parvo, kennel cough) can help to keep your pet's immune system strong. Animalsthat become sick as a result of parvovirus or other vaccinatable diseases becomestressed. This stress of illness suppresses their immune systems (and, in the case of parvo, damages their intestines), making it easier for coccidia to get a foothold.
Vaccine-induced immunity is strongest within the first 6 months of a vaccine and, therefore, vaccinating your dog a month or two before it meets other pets will reduce its riskof contracting a vaccinatable infectious disease.
2) Check out the boarding kennels or cattery for yourself before putting your pet in there:
Ensure that your pet's accommodation is suitable. Look for signs of poor husbandry and unsanitary conditionsthat might promote disease spread. Does the place smell OK? Is the place well-cleaned(look for excessive dustiness and dirt and faecal contamination). Are faecesable to travel from one cage to another under doors and walls? Is the place well-ventilated or is it musty and enclosed with stale-smelling air? Are dogs or cats placed in yards together (which could enhance disease spread)? Do the animals share food and water bowls? Are the food and water bowls clean? Is the place overcrowded with animals? Are animals placedin situations where they may become stressed (e.g. dogs and cats facing off against each otherthrough wire fences, cats all placed in together, extremes of heat and cold, inability to get dry, draughts etc.)? Look at the other pets in the place (if you are permitted to) - do you notice any coughing or sneezing animals or animals with watery orloose stools? If you see any major problems, don't board your pet there.
3) Only attend boarding kennels, groomers and dog clubs which have strict vaccine requirements:
If they are being strict on you with your pet's vaccinations, you can be surethat they are being strict on everyone else too. This strictness keeps disease out of a place. Lax rules result in diseases. Generally places with strict vaccine requirementsoften have high standards of hygiene and cleanliness within the facility too - thisis vital to keeping coccidiosis under control.
4) Don't board young puppies and kittens:
Young pups and kittens are the most prone to contracting clinical signs of coccidiosisand becoming very unwell. As hygienic as they try to be, kennels and catteries are oftenoverrun with adult animals that are non-clinical carriers of feline and canine coccidia. The faeces of these affected animalscan infect your young animal, making it very unwell.
5) Take your own food and water bowls to the boarding kennel, cattery or any dog shows or dog club events: This way your pet will not have to use another, possibly fecally contaminated, bowl.
When walking your pet, make sure that you keep him or her on a lead. This way, you willbe able to prevent your pet from running off and consuming the faeces of other animals. Never let your pet eat the droppings of other animals - poo contains lots of nastyorganisms that your pet might catch.
Similarly, do not let your pet hunt rodents or other animals and consume the uncooked meat oroffal from deceased animals. If you do like to feed your animal raw meat (especiallyraw bones - after all, bones should never be fed cooked), then make sure to freeze the meat for a minimumof 3 weeks before feeding it to your animal. This will kill any infectious coccidian tissuecysts. I do not advise feeding raw offal to dogs at any time (because of the risk of hydatidtapeworms), however, well-cooked offal (lung, liver, heart etc.) is fine.
When purchasing a pet for the first time, consider where your pet comes from. As mentionedin section 3, there are certain environments and facilities that are more likely to have high levels ofcoccidiosis and, thus, are more likely to sell you a coccidia-affected puppy or kitten. Petshops, shelters, pounds and poor-quality dog and cat breeders are more likelyto have coccidia on their premises. Make sure that, if you do buy a puppy or kitten fromone of these places (or any place, really), the kitten or puppy is well-grown (not stunted) andof good bodyweight and condition (a nice shiny coat and a bright, alert and interactive behavior). Do not buy sickly, lethargic, sleepy puppies and kittens, nor any that are underweightor which have poor coats or pot-bellies. Also make sure that any purchase comes with, at minimum, a 2 week wellness guarantee. It is not uncommon for puppies and kittens to seem fine inthe store and then break out with coccidia a few days after you bring them home becauseof the stress of moving-house.
Obviously, I do not want to put you off buying a pound or shelter animal - these animalsoften make great pets and are sorely in need of a loving forever home. Just make sure thatthe animal (dog or cat) is placed on a course of coccidial treatment (even if it notsymptomatic for the disease) prior to you taking it home. Also, make sure that the animal is well-vaccinatedand wormed prior to you taking it (remember that other intestinal diseases and parasites can makecoccidiosis worse).
Be aware of the potential for you or other people or animals to bring disease home to your pet. If you have visited a place where there has been coccidia infection (or any outbreak of infectious disease, for that matter), don't bring the disease back to your house on your hands or clothes, where your pet can catch it! Change your clothes, disinfect your shoes and shower thoroughly (this includes washing your hair)before greeting your pet. Ideally, disinfection should take place somewhere other thanyour own home. The same precautions should also be taken when other people visit your home:ask them if they have had any potential contact with infectious animals, puppies andkittens with diarrhea etc.
If you have had a case of coccidiosis in your own home and/or yard previously, then you will needto disinfect any contaminated areas and feed bowls in order to make them safe for any new puppiesand kittens that you bring home in the future (see section 11 below on coccidia decontamination). Coccidial organisms are resistant to many kinds of physical and chemical disinfection techniques and thorough cleaning is required to remove the organisms.
9b) Enhancing the health, body condition and immunity of the animal.
A big part of infectious disease prevention, particularly clinical coccidial disease prevention, requires the activity of a healthy, fully-functioning immune system. Although avoidanceof the infectious organisms (avoiding faecal-oral transmission and ingestion of infectedtissues and organs) is key to preventing the disease, the overall health and wellbeing of the animal is critical if there is to be a strong immune systemresponse towards the parasite, should your animal ingest it. Disease prevention involves a holistic approach, with nutrition, exercise and mental health care all important parts of the process. Animals that are in good body condition and which have been given a balanced diet and are well-exercised and emotionally sound (e.g. not overly stressed or bored etc.) will generally be more resistant to disease and able to mount a better immune response.Well-kept animals should not develop severe signs of coccidiosis (if any) and any period ofillness should be milder and more short-lived than that seen in poorly-cared-for pets.
Puppies and kittens that are malnourished and/or which harbour other bacterial and parasitic infectionsin their intestines are more prone to severe coccidial infections. Thus, goodnutrition and control of intestinal parasites (worming etc.) is important in preventing and reducing coccidian severity.
Some hints and tips:
- Puppies and kittens and adult dogs and cats should be routinely wormed.
- Puppies and kittens and adult dogs and cats should be fed a completely balanced (AAFCO approved) diet.
- Puppies and kittens should be played with often and exercised within the home until fully vaccinated(this human contact fulfills their exercise and mental health needs, but keeps them safefrom disease until fully vaccine protected).
- Dogs should be walked twice daily (once vaccinated) for a bare minimum of 15-30 minutes at a timeto aid both their mental health (reduces boredom) and exercise needs. Cats can be walked, butproviding them with an outdoor playpen (e.g. large cat aviary) can be enough to providethem with adequate exercise.
- Dogs and cats should have toys and adequate room to move (e.g. yard size, cat aviary) to reduce boredom and stresswhilst you are at work.
- Dogs and cats should not be overfed or allowed to become obese.
- Dogs and cats should not be kept in dusty, irritant or poorly-ventilated air conditions.
- Dogs and cats should be kept in low-stress, dry, clean, sanitary environments with no extremesof heat and cold, no draughts, good shelter and no build-up of urine or feces.
- Any known stressors should be identified and removed, if possible.
- Purchasing in-bred animals should be avoided - excessive in breeding may result in appearance of genetic immune disorders.
10. Controlling feline and canine coccidia in high-risk and/or already contaminated environments.
The following discussion mostly revolves around the control and management of Isospora - coccidiaspecies that are transmitted from dog to dog and cat to cat through the fecal oral route oftransmission. The control of other coccidian species (ones that require a dog or cat to ingestan intermediate host animal) is much simpler. These coccidian species can be simply controlledby feeding cats and dogs good quality commercial diets and well-cooked meat or offaland by preventing these animals from hunting live prey (rodent control is essential here) and scavenging for carrion.
Feline and canine coccidia outbreaks are quite common in large multiple-dog and multiple-cat facilities such as breeding facilities, shelters, pounds and even boarding kennels. Suchoutbreaks and endemic infestations in a premises can become highly costly in termsof treatment and isolation (staff care) of sickly animals; disinfection of infectedpremises; lost reputation and lost productivity (sickly, stunted puppies and kittens that can't be soldand reduced fertility of unwell breeding animals). Consequently, large dog and cat facilities need to have protocols in place that will help them to prevent coccidia and help them to deal with an outbreak of coccidiosis, should it occur. Control and prevention of disease in these situations involves:
a) protecting the susceptible animals and preventing them from becoming infected,
b) reducing environmental parasite oocyst contamination and destroying the infectious particles andc) reducing the non-infectious environmental and stress factors that can contribute to the disease.
10a: Protecting the susceptible animals.
In a situation of high coccidia risk or pre-existing contamination of the environmentwith infectious particles, any very young or very old animals; any unwell animals (animals with pre-existing disease); any stressed animals (e.g. pregnant, weaning, growing or lactating animals) and any animals of high-risk, immunocompromised breed-lines are going to potentially be susceptible to infection. The risk of these animals, and any other dogs and cats in the facility, contracting coccidia can be reducedin the following ways (this is by all means not an exhaustive list):
1) Ensure that all animals within the facility and allthose entering the facility are up to date with their vaccines.
Preventing animals from catching common, vaccine-preventable diseases can go someway towards keeping an animal's immune system strong. Animalsthat become sick as a result of parvovirus or other vaccinatable diseases becomestressed. This stress of illness suppresses their immune systems (and, in the case of parvo, damages their intestines), making it easier for coccidia to get a foothold.
2) Isolate pregnant bitches or queens and their litters from the rest of the dog and cat population.
Pregnant and lactating bitches are under massive levels of physiological stress and are, therefore, highly susceptibleto parasitic infections. Likewise, newborn puppies and kittens are also at risk of infection because their immune systems are not yet fully developed. Isolate bitches and queensand their litters in easy-to-disinfect (e.g. tiled or concrete areas, not grass or soil) pens,which have, preferably, never before been contaminated with coccidia*, from the time of whelping (queening) to the time of weaning. These pens should be located as far away as possible from the main dog population so that fecal run-off does not contaminate the bitch's/queen's quarters. If whelping bitches and queens can be removed to a new non-contaminated premises altogether, whiledecontamination of the coccidia-infected facilities is occurring, so much the better. All bedding and feed and water bowls provided should be new (or well-disinfected) and should be strongly disinfected or discarded after use by the bitch or queen. Steel bowls areeasier to disinfect (they can be autoclaved and boiled).
* Because of the high prevalence of non-clinical coccidia carriers, it can be very difficultto tell whether an area has ever been contaminated or not.
3) Pre-emptively treat bitches and queens with coccidiocides.
Pregnant bitches and queens (pregnant cats) can be treated with coccidiostatic drugsin the weeks just prior to whelping or shortly after whelping. This will greatly suppressand reduce the numbers of oocysts being shed by carrier mothers and thus reduce the risk of coccidiosis affectingthe highly-susceptible newborn litters.
4) Ensure that puppies and kittens get their colostrum on time (<24 hours after birth).
Puppies and kittens must be monitored to ensure that they do get their colostrum (maternal immunity).Bitches and queens that are recovering from caesarean section (unable to fed the young yet) or that areinitially reluctant to feed their offspring for other reasons can be milked of their colostrumand this can be fed to the pups and kittens through bottle or stomach tubing.
5) Don't expose puppies and kittens to contaminated environments.
Puppies and kittens should, ideally, go from the safe whelping zones straight to their new owners.The aim is to sell and get the young animals out of the infected environment ASAP. They should not come into close contact with the general canine and feline population, where coccidia sheddersare highly prevalent, nor should they come into contact with another animal's food or water bowls (which could be infected) or feces or any areas of the premises that are impossible to disinfect (lawns and soil). In extremely high-risk situations, early weaning (4-5 weeks instead of 6+ weeks) may be a consideration - try to get the pups and kittens out of the environment as early as possible.
6) Wear gloves.
Whelping bitches and queens and newborn puppies and kittens and other animals in high risk situationsshould be handled with gloves (plastic disposable aprons are also available for the extra-cautious to shield dogs and cats from possibly-contaminated clothing). New gloves and aprons should be used for every litter to avoid passing infectious organisms from one bitch/queen (and litter) to the next. If possible, gloves should be used when handling all other dogs and cats on the premises too (though, cost-wise this might not be practical in a big population).At a bare minimum, hands should be washed thoroughly after handling each dog or cat, prior tohandling the next.
7) Avoid in-breeding - i.e. don't breed susceptible animals.
In-breeding (e.g. breeding facilities) should be avoided to reduce the risk of genetic immune system disorders appearing and enhanced susceptibility to parasitic, viral and bacterial infections.
8) Control other easily-prevented diseases that might compromise an animal's gastrointestinal tract or immune system and make that animal susceptible to coccidial infection.
Gastrointestinal diseases (e.g. bacterial infections - Campylobacter and Salmonella) and high intestinal parasite burdens compromise the defensive mechanisms of the gastrointestinal system, reducing the ability of the bowel to clear coccidia and othersuch parasites. Additionally, low-grade, chronic diseases (including parasitism) can cause chronic, low-grade stress, which may suppress the immune system's response to coccidia. Consequently, control of these other parasites and diseases, through appropriate medications and parasite programs (routine worming etc.), is essential.
Good hygiene is crucial here too: ensuring that all runs, feed bowls and water dishes are cleaned promptly of fecal and respiratory tract secretions and ensuring that excrement and secretions are not permitted to spread to other runs or kennels through gutters and run-off. Mostintestinal parasites and bacterial and viral infections are spread through fecal-oral routes: less fecal contamination; fresh, clean bowls; regular cleaning of runs and cages andminimal fecal run-off between cages and runs (decent isolation of animals) means less disease transmission from animal to animal.
9) Avoid overcrowding of animals.
Overcrowding promotes rapid infectious disease spread from animal to animal; itpromotes stress which suppresses the immune responses of susceptible animals and overcrowding makes itdifficult for facility operators to identify the sick and to find a place, sufficiently out of the way, to isolate the sick, once they have been identified. With diseases like coccidiosis, overcrowding is of particular concern because faecesare more likely to missed by cleaners (not seen) and stood in or consumed by other animals.
10) Don't keep young animals in places with adult animals.
Adult animals often carry coccidia: they shed the infectious oocysts in their faeces, which can then be ingested by susceptible younger animals. Similarly, do not mix younger animalsof different ages or sizes in the one pen.
11) Consider placement of food and water bowls.
Feed and water bowls that become contaminated with faeces as a result of puppies andkittens walking through them (after walking through their own faeces), are an excellentsource of coccidia transmission from animal to animal. The animals can't avoid eating infected faeces if they are eating and drinking from infected food and water dishes.Contamination of food and water dishes can be reduced by elevating the dishes off the ground a bit(pups and kittens are more likely to walk around them then) and by placing the food and water dishesat the back of the run, away from the action. Pups and kittens always rush to the front of a cagein excitement when they see people and other animals - if the bowls are placed at the front of thepen, they will be stepped in during the mad scramble. If the bowls are placed at the back of the cage, the young animals will be running away from them - they will be less likely to be trodden in. Frequentcleaning and disinfection of food and water bowls is also important in coccidia control.
12) Reduce stress.
The number of susceptible animals is reduced when stress is low. This is discussedin section 10C - reducing the non-infectious, environmental and stress factors that can contribute to disease.
10b: Reducing environmental contamination with coccidia:
In any place where coccidia has appeared, it is important to ensure that contamination is minimizedwhere possible (e.g. reduce the number of infectious particles that enter a 'contaminated' area); that the oocystsare destroyed as thoroughly as possible (decontamination of the environment) and to ensurethat the infectious disease organisms do not spread from the contaminated regions to 'clean' regions of the premises and beyond.
1) Isolate infected or sick animals.
Because of the high prevalence of non-clinical coccidia shedders in the population, it can bevery difficult to completely remove coccidia-shedding animals from the generalanimal population. In the presence of coccidia carriers, there will always be some levelof oocyst contamination of the environment.
Sick animals, however, with clinically obviousdisease signs of coccidia, do, as a general rule, tend to shed greater numbers of oocysts in theirfaeces than non-clinical carrier animals do. The fact that these animals are displaying diseasesigns suggests that the level of coccidia replication and oocyst production is greaterin these animals than in the carriers. Consequently, removing obviously sick animalsfrom the general animal population will go some way towards reducing the overall degreeof coccidial contamination of the environment.
Sick animals should be removed from the general population and put into a quarantine region well away from the other, unaffected animals. Typically, severely affected pets will go to the vet and the vet will isolate them in a special 'iso' - isolation - ward. This quarantine region should not be near any thoroughfares (places where animals and people frequently walk) and well out of the way.
Animals in quarantine should be handled as little as possible (though not infrequently enoughthat they aren't getting proper care). When handled, the carer should wear disposablegloves and a disposable gown. All materials used by the carer should be disposed of in bins present within the iso ward, so that nothing that is potentially fecally contaminated andinfectious gets brought out into the 'clean' areas of the premises. Shoes are generally washed thoroughly in disinfectant foot baths (see section 11) as the carer leaves the isolation area, however, disposable shoe covers (covers worn over the shoes in the isolation area) can be used as an alternative.
2) Quarantine areas where sick animals have been.
Areas where sick animals have been should be quarantined from other animals until thoroughly disinfected (see section 11) and rested. Coccidia are quite resistantto a range of disinfectants and environmental conditions and so disinfection needsto be very thorough.
3) Discard contaminated bedding.
Any towels or bedding that the sick animal has been in contact with should be discarded as garbage once soiled. In the case of coccidiosis, you could disinfect and salvage thebedding if you really need it (e.g. if the bedding is really expensive or valuable), however, the best option is always to throw out contaminated items thatcould harbour potentially infectious, parasitic particles.
4) Be mindful of run-off.
Be careful that run-off from quarantined areas does not travel to 'clean' areasof the premises. Be mindful of gutters, gaps under the walls of pens and gaps in the walls of pens (e.g. wire fencing), that might allow fecal and diarrhea fluids from infected animals to contaminate clean areas or waterways. Be also aware of the 'sloping' of concrete or tiledruns: ensure that liquid run-off from one pen can not run down a slope and into another.
5) All infected rooms, pens and fomites (feeding dishes, grooming gear, benches etc.) should be disinfected thoroughly before exposing other animalsto them.
6) Avoid overcrowding of animals.
Overcrowding promotes rapid infectious disease spread from animal to animal; itpromotes stress which suppresses the immune response and overcrowding makes itdifficult for facility operators to find a place, sufficiently out of the way, to isolate the sick.
7) Consider your choice of surfaces: you want surfacesthat don't hold or protect bugs and which are easy to clean and disinfect.
It is almost impossible to keep infectious diseases and parasites out of dirt flooring andgrassy areas: such surfaces are difficult to decontaminate and they tend to pool water, which promotes the growth or survival of infectious organisms. In the case of coccidia, surface choice is very important. You can not easily get at dirt or grassy areas of a yard with disinfectants and, because coccidial oocysts are somewhat resistant to heat, sunlight,freezing conditions and drying conditions (desiccation), the oocysts will remain infectiveto other animals for many months in soil and lawn.
Author's note: Coccidian oocysts do eventually die from sunlight, heat and desiccation, butthis can take weeks to months. Oocysts (the infectious stages shed in the poo) will survive freezing environmental temperatures for very long periods, even though the infective cysts found in the meat and offal of intermediate hosts do not.
It is far quicker and easier to rid your premises of coccidia (or at least reduce coccidial contamination)if you can thoroughly disinfect your premises from top to tail. For ease of disinfection (including parvo etc.), you are better to go with cement or tile flooring, which can be easily cleaned of feces, urine, vomit and respiratory secretions and readily disinfected.
Choice of fencing/walls is important too. Smooth-textured (e.g. steel), solid, filled in wallsare easier to clean than wires or rough brickwork (which have a hugely increased surfacearea for parasites, bacteria and viruses and feces to cling to). Solid, filled in walls also promote lessaerosol and faecal transmission of disease from dog to dog or cat to cat than open wiring does.
8) Routine cleaning is a must.
Routine sanitation and cleaning is a must in all dog and cat facilities. All surfaces should be routinely disinfected and kept clean. Feces and urine must be removed daily to twice dailyat a bare minimum. Routine spraying of runs with water to remove particulate matter and dust is important (these harbour infectious organisms and bacteria) andintermittent (weekly to 2-weekly) steam-cleaning of surfaces is useful to reduce infectiousdisease loads (even parvo is killed by steam-cleaning).
Don't forget to clean cage or door bars and vertical walls where an animal might havesmeared faeces.
9) New animals to a premises should be quarantined and given coccidiostats.
As a routine part of infectious disease prevention, all new dogs and cats to a breeding facilityor pound, shelter or boarding kennel should be quarantined away from the main dog or cat population for at least a month. The animals should be fully wormed and vaccinated and monitored for disease appearance. Only when the time of quarantine is up and the animal considered free from disease, should he or she be allowed into the greater animal population. Most animals withcoccidiosis should manifest obvious disease symptoms within this month, due to the stressesassociated with their relocation.
Note that this quarantine zone should not be an area that has had diseased animalsin it before, if possible. If the quarantine zone has had diseased animals in it previously, then it should have at least been thoroughly disinfected and spelled prior to the arrival of the new animal for quarantine. If not, the new non-diseased pet could contract diseases from the quarantine area.
Quarantined animals should be treated like isolation animals, with gloves, gownsand foot bathing (or shoe covers etc) used to prevent disease going from thequarantine zone into the rest of the population or from the rest of the population into the quarantine zone.
Animals newly admitted to a premises are usually vaccinated and wormed pre-emptively, however, they can also be treated with a coccidiostat too. Most anti-coccidial drugs are quitesafe and cheap to use and can be given to puppies and kittens and new arrivals in the absence of anyclinical symptoms. A lot of shelters routinely treat their new kitten and puppy arrivalswith coccidiostats in order to keep coccidiosis to a minimum.
In theory, households looking to introduce new animals into their already existent pet family could also employ a similar quarantine procedure. If this is notpossible or practical, new additions to the family should ideally be bought from reputable breeders or families (places with excellent standardsof pet care and hygiene) and vaccinated and wormed and given a coccidiocide (e.g. Baycox for three days)prior to arrival.
10) Staff should know the signs of unwellness and coccidia infection in puppies and kittens.
Early detection of illness is key to establishing rapid isolation of an animaland reducing the spread of diseases like coccidia. Staff should look out for lethargic, inappetent (or reduced appetite puppies) or 'loner' (puppies on their own) puppies and kittens. Puppies and kitties with any diarrhea, blood in the stools or excessively smelly or mucoid droppings should be immediately isolated and treated. Recognition of puppies in poor condition or unwell condition is very important in places like pet shops that receive lots of unvaccinated and unwormed puppies through their doors. These places should not take on any weak or sickly puppies to sell, as they risk contaminating their facilities and all the other healthy pups thatthey get in.
11) Good record keeping.
Breeders that receive dogs and cats from elsewhere and pet shops that receive lots of for-salepuppies and kitties from various sources must keep good records of where these animals have come from. Good record keeping allows these facilities to recognise breeders and sellers thatare frequent offenders and who frequently sell underweight, malnourished, wormy or coccidia-affected animals. Once identified, not buying from such places can go a big way towards preventing coccidiosis and other contagions fromgetting into your facilities.
Good record keeping is also essential within large facilities such as pounds, sheltersand breeding kennels and catteries. Noting which runs and groups of cages tend toget the highest numbers of coccidia infected animals can be very valuable. If certaincages and dog runs tend to be frequent offenders, this lets operators know that thereis a problem to be solved in that area. Is the staff member responsible for cleaningthe cages not doing his/her job? Is the cage excessively draughty and cold and stressful? Does the run slope oddly, allowing for fecal spread and contamination? Is there a significantinsect or rodent source of infection? Does water tend to pool in those runs?
12) Control rodents and insects.
Rodents and insects can potentially carry coccidia organisms on their feet from animal to animal and place to place. Flies are a particular problem because theyare attracted to faeces and flit from dropping to dropping and cage to cage.Strict control of these vectors must be put into place in order to reduce the spread of coccidia in a facility. Obviously, removal of factors which attract rodents (such as grass, grain, open dog-food packs and warm, hidden areas to breed) and flies (feces, open foodstuffs) is vital, but adequate control may need to include the use of rodent poisons and insecticides and 'fly traps'.
10c: Reduce the non-infectious, environmental and stress factors that can contribute to coccidiosis.
Stress lowers the immune system defenses of animals, making them more susceptible to showing diseasesymptoms. Each point below addresses a different factor in stress-management:
Ensure that all animals have adequate nutrition. Happy, well-nourishedanimals of good bodyweight generally have a better immune system and are better equipped to cope with disease.Remember to treat each animal as an individual: regularly record the weight and body conditionof each puppy or kitten to ensure that the nutrition provided is adequate for him or her andto ensure that the animal is actually getting fed. It is easy in crowded situationsfor individual animals to be underfed (as an individual, they might simply need more food than is on offer) or for carers not to realise that a puppy or kitten is being bullied out of a meal. Rememberthat nutritional monitoring is particularly important in animals that have highmetabolic requirements: pregnant dogs and cats, lactating dogs and cats, growing pups and kits and performanceanimals (e.g. working dogs, sporting dogs and obedience dogs).
Temperature and moisture control. Making sure that kennels, cages and runs are warm and dry and free of draughts and chillswill also go a ways towards keeping animals comfortable and stress-free. Temperature controlin runs is important: excessively cold and hot conditions are stressful and can facilitate disease. Constantly fluctuating temperatures are also stressful, even if the swings are not extreme: maintaining a steady-state, comfortable temperatureis ideal. According to Greene CE, temperatures in kennels should be between 21-24 degrees-C. Remember that temperature control is particularly important in animals that have other stresses (you don't want to add temperature issues to their worries): pregnant animals, lactating dogs and queens, growing pups and kittens and performance animals (e.g. working dogs, show dogs and cats, sporting dogs and obedience dogs).
Sanitation. Making sure that kennels, catteries and runs are cleaned and sanitary goes a long way towards reducing stress. Kennels and runs must be cleaned twice to three-times daily (or more, if possible). Animalsdo not like sitting in feces and urine any more than you do. Excrement promotes thespread of coccidia and also the growth and spread of other diseases and infections that the animal's immune system must then fight (e.g. bacterial infections). These 'extra' infections divert a portion of the animal's immune system away from fighting diseases such as coccidiosis or parvovirus, which the animal might then, as a result, contract (i.e. the animal'simmune system may have been able to defeat the coccidia or parvo organisms, hadit not also been engaged in fighting off the diseases caused by the lack of sanitation). Remember that good sanitation is particularly important in animals that have other stresses (you don't want to add sanitation issues to their worries): pregnant pets, lactating breeders, growing puppies and kittens and performance animals (e.g. working dogs, sporting dogs and obedience dogs).
Exercise and mental stimulation. Animals get stressed when they are bored andunstimulated and have no opportunity to occupy themselves. Dogs and felines are intelligent creatureswhich need stimulation, both mentally and physically. Dogs in large dog facilitiesshould be walked a minimum of twice daily (ideally for 30 minutes or more at a time)and be given room to run around in and toys or other nice dogs to play with (provided theyall get on OK). Cats should be provided with ample space to run around in and toys and climbing framesand other nice cats to play with (provided they all get on OK). Bitches and queens with newborn litters generally don't like to interact with other dogs or catsor to be taken away from their offspring and so they should be offered toys and a moderate amountof space and allowed to choose their activities for themselves.
Stress-relief for late-pregnant and lactating bitches and queens. In the wild, these animals usually go off on their own (into a dark den) to give birth and to rear their litters in private (at least for the first 6 weeks). In a breeding facility, these animals should be allowed their privacy: a warm, well-beddeddimly-lit place to give birth and rear their pups and kits. These animals should not be placedin close proximity (sight, smell or hearing) to other dogs and cats and should not be forcedto socialize or leave the newborns too often. This causes stress.
Avoid overcrowding. Yes, I know I have mentioned it 3 times now. This shows how important I think itis in the prevention of coccidiosis and other diseases. Overcrowding promotes rapid infectious disease spread from animal to animal; itpromotes stress which suppresses the immune response of susceptible animals and overcrowding makes itdifficult for facility operators to find a place, sufficiently out of the way, to isolate the sick.
Be aware of dog-to-dog and cat-to-cat and cat-to-dog interactions. Some animals do not like being housed near other animals for many reasons: simple dislike, prior experiences, the other dog or cat making threatening gestures at them through the fence (rigid stance, growling, staring etc). Placing an animalfor long periods of time in close proximity to an animal that it fears or does not get alongwith will inevitably lead to stress. Dogs and cats may not realise (as we do) that the big dognext door can not get at them through the fence. Thus, leaving those animals sideby side (especially in wire-fenced cages where they can clearly eye each other) willresult in one animal being in constant fear that the other will soon attack them.Ideally, only place animals in close proximity that get along or are at least indifferent to each other.
11. How to disinfect the environment following feline and canine coccidia contamination.
Regardless of whether an affected coccidia case appears in your own home or in a larger multiple-dog or multiple-cat environment such as a breeding facility or veterinary clinic, it is important that correct measures are taken to clean and disinfect the contaminated areas.
Chemical agents (disinfectants) used to kill coccidia organisms.
Coccidial oocysts that contaminate an environment are notoriously resistant to most disinfectant chemicals. In fact, some texts even go so far as saying that there is nochemical disinfectant that is 100% reliable at completely removing infective coccidial oocystsfrom an environment and that all we can hope for is for the disinfectant to reduce the numbers of oocysts down to minimally infectious levels (reduce the environmentalburden). The following sections list the chemicals and protocols that have been found to have someeffect at killing coccidial organisms (including Toxoplasma).
Initial cleaning with water.
Before a disinfectant can be used on an area, it is important to thoroughly scrub thearea with water beforehand. This is to remove any gross pus, fecal, urine, vomit and soil matter from the surfaces. It is important to do this because organic matter such as feces can inhibit the effects of some disinfectants (for example QUATs)and even deactivate them! Removing fecal and soil/dust matter will also reduce the surface area over which the coccidia oocysts can hide out, thereby enhancing the ability of the disinfectants to make contact with them and kill them.
This initial cleaning process should not contain disinfectants or other soaps because somedisinfectants will react badly with or deactivate other kinds of disinfectants (the reaction can even create poisonous gasses which you might breathe in).
Most texts that discuss the disinfection of coccidia contamination in dog, cat and livestock facilities recommend that a solution of 10% ammonia (a very high concentration) be appliedto infected surfaces. This solution can be left to dry and stand overnight.
Ammonia solutions release nasty, irritant, noxious vapors that irritate the airways, nasal passages and lungs of people and animals. Ammonia should, ideally, only be used in well-ventilated areas or, if used in poorly ventilated areas, with appropriate safety apparatus (face and eye masks, possibly even breathing apparatus). High ammoniaconcentrations can also cause nasty chemical burns if animals are directly exposed tothem. Animals must never be allowed to consume ammonia.
Once applied and given time to act (e.g. left overnight), ammonia solutions must be rinsed thoroughly, leaving no residues, and the area allowed to ventilate well before animals can be reintroduced.
The phenolic compounds have also been suggested in some texts as a way of decontaminating coccidia-affected premises. Phenols are of particularbenefit in kennelling and cattery situations since they are not deactivated by other soaps or organicsoil and faecal matter. Like most of the disinfectants discussed on this page, phenoliccompounds can leave skin and mucous-membrane irritant residues: surfaces cleaned withthem should be rinsed thoroughly after application (prior to reintroduction of animals). This is particularly important in feline premises and premises with large populations of neonates (newborns): catsand newborn litters are very susceptible to the toxic effects of phenolics.
For those of you looking for effective, biodegradable, non-toxic chemicals, there is a new compound put out by the Nutribiotic company called Citricidal (ref. Greene CE). This is a natural diphenol derived from grapefruitseed.
Non-chemical agents used to kill coccidia.
The best way to kill coccidial organisms is with heat: boiling water or steam or sunlight(though natural sunlight exposure takes weeks to months to kill coccidia). Most ofthe texts that discuss coccidial decontamination of a premises suggest using steam cleaning orboiling water as the main method of decontamination. Not only are these techniques the most effective means of reducing oocyst numbers, they are also devoid of harmful residues and vapours.
Most infectious disease organisms, including resistant heavy-weights such as parvovirusand coccidia, are susceptible to the effects of intense heat and pressure. Steam applied underpressure (steam cleaning) is the most effective means of achieving sterility (Greene). Steam cleaning can be used in cages, dog runs, concreted areas and tiled regions and may be an option for carpets, clothing and bedding where other chemical compounds might not be appropriate. Autoclaving (whereby hospitals and vet clinics use intense pressure and heat to sterilize surgical equipment) is a good way of removing infectious organisms from steel fomites, instruments and cloth materials.
Bowman (reference 13) recommends the following method for decontaminating farrowingcrates (piggery cages), a technique which could easily be applied to dog and cat facilities:steam clean the farrowing crates; then wet the crates down with a 10% ammoniaor phenolic disinfectant compound; let it stand overnight and then rinse the cratesthoroughly and repeat the steam cleaning again the next day.
Boiling your drinking water has long been a tried and true method of destroying parasitespresent in natural waterways (e.g. Cryptosporidium, Giardia) and fouled drinking water supplies.Immersing steel fomites (e.g. dog bowls, water dishes), surgical instruments and fecally contaminated clothing in rolling boiling water for 15-30 minutes should kill any coccidial organisms present. It is a simple, easily available, cheap and effectiveway of removing infectious coccidial organisms from steel fomites, instruments and cloth materials.
Depopulation and resting the environment.
Sunlight, heat and dry, desiccating conditions will eventually kill coccidial organisms, although this takes time. Simply removing all animals from a premises, disinfecting the premises well and leaving the premises unpopulated for several months maybe a useful way of reducing coccidial contamination. This technique will probably not work in cold or freezingclimates because oocysts are very resistant to freezing.
Remove the infected surfaces.
This is an expensive way to remove infectious disease organisms, however it does work. Routinely replacing gravel, topsoil or lawn surfaces that have become contaminated and replacing them with new, fresh, non-contaminated surfaces is an excellent way toremove infectious disease organisms, particularly long-lived organisms such as parvovirusand coccidia. If the difficult-to-clean surfaces (soil, lawn etc) can be replaced with new, easy-clean surfaces such as concrete or tiles ormetal (in the case of runs and cages), so much the better.
12. Removing coccidia cysts from contaminated meat:
Infectious coccidia cysts (including the cysts of zoonotic, highly-infectious, coccidia-related organisms such asToxoplasma) can be contracted by dogs and cats (and man) through the consumption of undercooked meatand offal (particularly the liver and brains). A wide range of wild and livestock animalspecies commonly fed to pets by their owners can carry these cysts, including cattle, sheep, goats, pigs, deer, kangaroos and horses.
Owners can prevent their pets from ingesting meat-borne coccidial cysts by not feeding theirpets raw meat or offal. Thorough cooking destroys the cysts, along with a range of other nasty, meat-associated, bacterial and parasitic pathogens, and is the safest way of feedingmeat or offal to animals.
Owners that prefer (or insist upon) feeding raw meat productsto their animals should only purchase human-quality meat products for their pets. These meat and offal products have been thoroughly checked out by abattoir officers and deemed safe for human (and pet) consumption. Such abattoir-approved products should, hopefully,not contain any nasties such as hydatid cysts. Owners should never feedself-killed, raw meat or offal to their pets (e.g. kangaroos and sheep killed throughhunting activities) because most owners are not skilled at recognizing diseasedorgans and muscles and might inadvertently feed these to their pets.
Any meat, bones and offal purchased for animal consumption (that owners plan not to cook) should befrozen in the freezer for a minimum of 3 weeks before defrosting them and feeding them to their pets. Freezing will kill any encysted coccidial parasite cysts.
13. Coccidia and coccidiosis - a summary of the important points:
1) Coccidiosis is a common, highly contagious disease characterized by watery to custard-consistency (mucoid) diarrhoeaand the production of mucoid, jelly-like faeces with or without the presence of blood (hematochezia). Animals with non-complicated cases of coccidiosis are usually bright and alert and well-looking and still eating. Vomiting is not a typical presenting sign, but can be present.
2) Coccidiosis is caused by a number of infectious protozoan organisms, Isospora being the most common.
3) Coccidia transmission typically occurs when individual dogs and cats are introduced into environmentswith large numbers of other dogs and cats (e.g. kennels, pounds, shelters etc.). The disease is normally spread by the consumption of faeces and fecally contaminated foods, water bowlsand surfaces. Certain species of coccidia are transmitted to dogs and cats through the consumption of non-cooked herbivore or vermin meat and innards (offal or organs).
4) Coccidia are generally host-specific, seldom affecting species of animalsthat are unrelated to their usual definitive and intermediate host animal species.
5) Coccidiosis is exacerbated by a range of non-infectious disease factors, includingstress and immunosuppressive diseases and drugs.
6) Coccidiosis (the clinical disease) can become complicated by secondary bacteria, leading to severecases of bacterial enteritis and sepsis especially in young or severely immune-suppressed animals.
7) Coccidiosis is normally diagnosed on fecal floatation.
8) Treatment for coccidiosis involves giving the affected animal anticoccidial drugs and other supportive remedies and medications (antibacterial antibiotics, intravenous fluids etc.)and isolating the animal during the clinical stages of infection.
9) Coccidial drugs may not cure the animal of the parasite. Often the coccidiostatic drugs only act to suppress the symptoms of coccidiosis (shorten the period of clinical disease) until the animal's own immune system responds and suppresses the infection. It is common for a carrier state to develop, whereby the host's immune system suppresses the organism's replication (stopsit from producing clinical signs), but does not clear it from the body.
10) Non-clinical carriers of coccidia are common in the dog and cat population. They shed infectiveoocysts into their faeces, which are infective to other, susceptible animals.
11) Adult animals with recurrent or persistent (chronic) symptoms of coccidiosisshould be examined for an underlying immune suppressive disease or an alternative intestinaldisease that is either mimicking coccidiosis or exacerbating a pre-existing coccidial diseasecondition.
12) It is rare for coccidia infected animals to die from the disease. Young puppies, very old animals, animals with pre-existing debilitation and immune suppressed animalsare most at risk of secondary complications that could result in severe disease and even death.
13) No vaccine is available for coccidia.
14) Good sanitation and hygiene practices are essential to preventing and controllingoutbreaks and endemics of these organisms.
15) Coccidiosis is quite resistant to a wide range of disinfectants and environmental conditions. Steam cleaning is the best way of removingcoccidia from a premises.
16) Sarcocystis neurona is a newly recognised (emerging) coccidial disease that affects the brains ofhorses and cats.
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References and Suggested Readings:
1) General Therapeutic Principles. In Nelson RW, Couto CG, editors: Small Animal Internal Medicine, Sydney, 1998, Mosby Inc.
2) Dubey JP, Greene CE,Enteric Coccidiosis. In Greene CE, editor: Infectious Diseases of the Dog and Cat, St. Louis, 2006, Saunders Elsevier.
3) Greene CE, Ewing SA, Antiprotozoal Chemotherapy. In Greene CE, editor: Infectious Diseases of the Dog and Cat, St. Louis, 2006, Saunders Elsevier.
4) Greene CE, Environmental Factors in Infectious Disease. In Greene CE, editor: Infectious Diseases of the Dog and Cat, St. Louis, 2006, Saunders Elsevier.
5) Lawler DF, Prevention and Management of Infection in Kennels. In Greene CE, editor: Infectious Diseases of the Dog and Cat, St. Louis, 2006, Saunders Elsevier.
6) Greene CE, Gastrointestinal and Intraabdominal Infections. In Greene CE, editor: Infectious Diseases of the Dog and Cat, St. Louis, 2006, Saunders Elsevier.
7) Little SE, Laboratory Diagnosis of Protozoal Infections. In Greene CE, editor: Infectious Diseases of the Dog and Cat, St. Louis, 2006, Saunders Elsevier.
8) Greene CE, Watson ADJ, Antibacterial Chemotherapy. In Greene CE, editor: Infectious Diseases of the Dog and Cat, St. Louis, 2006, Saunders Elsevier.
9) Antimicrobial Drugs. In Carter GR, Chengappa MM, Roberts AW editors: Essentials of Veterinary Microbiology, USA, 1995, Williams and Wilkins.
10) Sterilisation and Disinfection. In Carter GR, Chengappa MM, Roberts AW editors: Essentials of Veterinary Microbiology, USA, 1995, Williams and Wilkins.
11) Host-Parasite Relationships. In Carter GR, Chengappa MM, Roberts AW editors: Essentials of Veterinary Microbiology, USA, 1995, Williams and Wilkins.
12) Sources and Transmission of Infectious Agents. In Carter GR, Chengappa MM, Roberts AW editors: Essentials of Veterinary Microbiology, USA, 1995, Williams and Wilkins.
13) Protozoans. In Bowman DD, Lynn RC, Eberhard ML editors: Parasitology for Veterinarians, USA, 2003, Elsevier Science.
14) Diagnostic Parasitology. In Bowman DD, Lynn RC, Eberhard ML editors: Parasitology for Veterinarians, USA, 2003, Elsevier Science.
15) Hulst F, Amphibian Care and Medical Management. In Wildlife in Australia: Healthcare and Management - PGF Proceedings 327, Sydney, 1999, Post Graduate Foundation in Veterinary Science.
16) Booth R, Monotremes. In Wildlife in Australia: Healthcare and Management - PGF Proceedings 327, Sydney, 1999, Post Graduate Foundation in Veterinary Science.
17) Lynch M, Pinnipeds. In Wildlife in Australia: Healthcare and Management - PGF Proceedings 327, Sydney, 1999, Post Graduate Foundation in Veterinary Science.
18) Rose K, Common Diseases of Urban Wildlife. In Wildlife in Australia: Healthcare and Management - PGF Proceedings 327, Sydney, 1999, Post Graduate Foundation in Veterinary Science.
19) Bellamy, T First Aid for Native Flora. In Wildlife in Australia: Healthcare and Management - PGF Proceedings 327, Sydney, 1999, Post Graduate Foundation in Veterinary Science.
20) Blyde D, Advances in Treating Diseases of Macropods. In Wildlife in Australia: Healthcare and Management - PGF Proceedings 327, Sydney, 1999, Post Graduate Foundation in Veterinary Science.
21) Disorders of the Intestinal Tract. In Nelson RW, Couto CG, editors: Small Animal Internal Medicine, Sydney, 1998, Mosby Inc.
22) Hall EJ, Simpson KW, Diseases of the Small Intestine. In Ettinger SJ, Feldman EC, editors: Textbook of VeterinaryInternal Medicine, Sydney, 2000, WB Saunders Company.
23) Lappin MR, Protozoal and Miscellaneous Infections. In Ettinger SJ, Feldman EC, editors: Textbook of VeterinaryInternal Medicine, Sydney, 2000, WB Saunders Company.
24) Levy J, The Fading Kitten and Neonate. In Rand J, editor: Problem-based Feline Medicine, Sydney, 2006, Elsevier Saunders.
25) Zoran DL, The Cat with Signs of Acute Small Bowel Diarrhea. In Rand J, editor: Problem-based Feline Medicine, Sydney, 2006, Elsevier Saunders.
26) Zoran DL, The Cat with Signs of Chronic Small Bowel Diarrhea. In Rand J, editor: Problem-based Feline Medicine, Sydney, 2006, Elsevier Saunders.
27) Diseases and Clinical Syndromes. In Tilley LP, Smith FWK: The 5 Minute Veterinary Consult, Baltimore, 1997, Williams and Wilkins.
29) Antimicrobial Drug Formulary - Appendix 8. In Greene CE, editor: Infectious Diseases of the Dog and Cat, St. Louis, 2006, Saunders Elsevier.
30) Amprolium. In Plumb DC: Plumb's Veterinary Drug Handbook, 5th ed. USA, 2005, Blackwell Publishing.
31) Furazolidone. In Plumb DC: Plumb's Veterinary Drug Handbook, 5th ed. USA, 2005, Blackwell Publishing.
32) Quinacrine. In Plumb DC: Plumb's Veterinary Drug Handbook, 5th ed. USA, 2005, Blackwell Publishing.
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Please note: the aforementioned coccidial treatment schedules and coccidialprevention guidelines and information on the coccidial diseases of dogs and cats are general recommendations only. The information provided is based on published information and recommendations made available from the drug companies themselves; relevant veterinary literatureand publications and my own experience as a practicing veterinarian.The advice given is appropriate to the vast majority of pet owners, however, giventhe large range of drug types and prevention protocols now available, owners should take it upon themselves to ask their own veterinarian what treatment and disease prevention schedules s/he is using so asto be certain what to do. Owners with specific circumstances (high coccidiacontamination in their environment, pregnant bitches and queens, breeders, multiple-dog and cat environments,animals on immune-suppressant medicines, animals with immunosuppressant diseases or conditions etc. etc.) should ask their vet what the safest and most effective protocol is for their situation.
Any dose rates mentioned on these pages should be confirmed by a vet. Dosing rates for commondrugs are being changed and updated all the time (e.g. as new research comes in and as drugformulations change) and information here may not remain current for long. What's more, although we try very hard to maintain the accuracy of our information, typos and oversights do occur. Please check with your vet before dosing any pet any medication or drug.
Common coccidia misspellings:
cocsidia, cocsidiosis, cocksidia, cocksidiosis, cokcidia, cokcidiosis, coksidia, coksidiosis, coccidyosis, coccidya, cocsidya, cocsidyosis, cocksidya, cocksidyosis.